Study On The Effect Of Dietary Supplement Of L-theanine On Anti Stress In Weaned Piglets

Stress has become set intensive culture mode of pig health hazards of important environmental cause. Chemical drugs to relieve or weakening of porcine stress harm will bring many negative effects.For biogenic and low negative effect of anti stress drugs to alleviate the modern pig model under the generated stress has important practical significance. Test to extracted from tea leaves of L-theanine as rawmaterials of theutility of anti stress ofweaned piglets, for practical applications providethe basis for.Experiment 1 Study on the construction of oxidative stress model induced by Diquat in weaned pigletsThis experiment was conducted by observing the changes of growth performance, serum biochemical and antioxidant indices in weaned piglets after disposable intraperitoneal injection of Diquat to verify whether the oxidative stress model was successfully constructed,and, further exploration on sensitive indicator of evaluating oxidative stress could achieve. A total of 10 DLY weaned piglets with a similar average body weight(10.64±1.08 kg) were randomly allocated to two groups, each group consisting of five replicates with one piglet.The disposable intraperitoneal injection with Diquat solution by 10 mg/kg bw was implemented in treatment group, and, the control group were injected with the same dose of sterile saline. During the 21-day experimental period, venous blood samples were colected before fasting at the 3rd, 10 th, 21 st day to determine the serum biochemical and antioxidant indexes. The results showed that, Diquat significantly decreased ADG and ADFI(P<0.01) of experimental piglets, significantly increased serum ALT, AST activity and COR content(P<0.05), and significantly reduce serum GSH-Px vitality(P<0.05). The experiment demonstrated that disposable intraperitoneal injection of 10 mg/kg bw Diquat solution could induce oxidative stress in weaned piglets, and, stress effect could maintain more than 21 days.What’s more, serum COR content and GSH-Px, ALT, AST vitality could be regarded as stress-sensitive indicators.Experiment 2 Effects of L-theanine on growth performance and serum biochemical indexes of oxidativestress weaned pigletsThe oxidative stress model induced by Diquat was used to study the effect of L-theanine on growth performance, antioxidant capacity and immune function of weaned piglets. 40 weaned piglets(11.62±1.32 kg) were divided into 4 treatment groups which were the controlgroup(basal diet + saline), L-theanine treatment group(1 000 mg/kg L-theanine diet + saline),stress model group(basal diet + injection Diquat), L-theanine handle stress model group(1000 mg/kg L-theanine diet + injection Diquat). Each treatment had 10 replicates, each with one piglet, and, the trial period was 21 days. Blood samples were collected at day 3, 10 and 21,and the growth performance, serum biochemical indexes, serum oxidative indexes and immune factors content were measured. The results illustrated that oxidative stress significantly decreased the growth performance, antioxidant capacity and immune function of piglets(P<0.05). Dietary supplemented with 1000 mg/kg L-theanine significantly increased the average daily gain and feed intake in stress group(P<0.05). Serum GLU, Ig A, Ig M, Ig G content significantly increased(P<0.05), serum COR, MDA, IL-10 content significantly decreased(P<0.05), and, serum GSH-Px activity and total protein content was on the rise(P>0.05).Test conclusion: weaned piglets by intraperitoneal injection of 10 mg / kg body weight dose solution Diquat can induce oxidative stress and the effect lasted for 21 days. Diets added1 000 mg/kg of L-theanine could reduce the oxidative stress of weaned pigs on the growth performance, antioxidation and immune function of damage effect.