Research On Cross Breeding In Three Species Of Rose And Genetic Transformation RrFLS In Rosa Hybrida ‘Samantha’

R.multiflora, R.chinensis and R.rugosa as the Chinese original Rosaceae(Rosa L.)are the important representative plant. They have a long cross breeding history and possess abundant gene source, which make them the important parents of cross breeding and the source of excellent traits gene as well as genetic transformation receptor.Accordingly, they have the value of garden ornamental and commercial application. In this study, Introduction of RrFLS and RrANR gene into somatic embryos of R. hybrida‘Samantha’ and make the Rr FLS and RrANR transgenic plants flower color turned into white from red, which based on the established Agrobacterium-mediated transformation system of somatic embryogenesis in R. hybrida ‘Samantha’. In addition, we study Rosa breeding for reference by interspecific hybriding between the R.chinensis and the R.multiflora and hybriding between different varieties of R.rugosa.1. Interspecific hybriding between the R.chinensis and the R.multiflora.The hybridized combination between the R.chinensis and the R.multiflora has 10 group. Fructification rate of all the combination is significantly different, in which of BQ×YF, 36.6±7.1%, is significantly higer than the others. Seed of per fruit of all the combination is also significantly different, in which of YZ×FQ and BQ×YF, more than10, is significantly more than the others. Comprehensive analysis showed that fructification rate and seed number of per fruit is effected by female parent significantly.YZ×FQ、YF×FQ、BQ×YF 、FQ×YZ are better hybridized combination. In addition to, 100mg/L GA3 is likely to contribute to increasing the fruit setting.Accordingly, SRAP analysis the10 hybrid progeny finding that two of YZ×FQ, two of YZ×FQ and YF×HQ are true hybrids.2. Hybriding between different varieties of R.rugosa.The hybrid combination has 6 groups between different varieties of R.rugosa.Fructification rate of all the combination is significantly different, in which DH as female parent, 69.84±9.84 w, 73.81±3.81 respectively, is significantly higher than the others.Seed of per fruit of all the combination is also significantly different, in which of both DH and DB as femal parent, the mean is 69.95±9.95 e, are significantly more than theothers. Comprehensive analysis showed that DH as femal parent gather more hybrid progeny. In addition to, based on the study of the 92 of DH×ZZ, we found that the hybrid progeny happened segregation of stem color, separation ratio of red to green to red in green is 1:1:2. Therefore, we deduced that the genetic approach of the color of stem was incomplete dominant inheritance in R.rugosa.3. Enhancement of somatic embryogenesis matureFor enhance the state of somatic embryogenesis and the degenerated callus, we study the influence of the different level of concentration of GA3 and TDZ finding that the 0.5 mg/L GA3 can accelerate mature of somatic embryogenesis, the best maturation medium: 1/2MS+1.0mg/L BA+0.01 mg/L NAA+ 0.5 mg/L GA3; the best maturation medium for the degenerated callus: 1/2MS+1.0mg/L BA+0.01 mg/L NAA+ 0.1 mg/L GA3+1.0 mg/LTDZ. In order to improve the growth condition of buds,we found that adding a strong bud part between germination and rooting is good for the growth of bud,and the medium for the buds less than 1 cm: 1/2MS+1.0 mg/L BA+0.01 mg/L NAA+ 0.1mg/L GA3; the medium for the buds higher than 1cm: MS+0.5 mg/L BA+0.01 mg/L NAA.