Study Of VOCs And Structural Changes In Rice Straw Degradation By Co-culturing Of Three Fungi

China owned the highest yield of rice straw in the world. As a simple, cost-effective and sustainable method to degrade the rice straw, the microbial degradation of rice straw has received widely attention and study. Many fungi can produce a high production of cellobiohydrolase enzyme to degrade lignocellulose, and many researches about the cooperation of different fungi in mixed cultures have obtained extensive attention. But the Volatile Organic Compoundsits(VOCs) produced by co-culturing in rice straw degradation still received little research in China, as well the compition in mixed cultures. There are many kinds of fungi can produce monoterpenes which may conduct an antagonism mechanism. On the one hand, the compition between fungi may inhabit the growth of other fungi, while on the other hand, it may stimulate the growth of fungis in mixed fungi.In this experiment, three fungi Pleurotus ostreatus, Aspergillus fumigatus, and Trichoderma viride were inoculated in rice straw powder to make the mixed culture, and the pure cultures of them were set up respectively in this study. Two aspects of analyzation and comparison was carried out among the three culture systems. The category number of VOCs and the content of monoterpenes were compared, and the ctructual changes of rice straw were observed by SEM. The results showed that:(1) Trichoderma viride produced more VOCs in single cultures among three fungi, and oberseved by SEM, Trichoderma viride degraded rice straw more compeletely.(2) The co-culturing of Aspergillus fumigatus and Trichoderma viride produced more VOCs and monoterpenes in the degradation of rice straw, it may be the result of competition in co-culturing, which caused the higher effecience of lignocelluloses degradation.(3) The co-culturing of Pleurotus ostreatus and Trichoderma viride produced more VOCs than Pleurotus ostreatus but less than Trichoderma viride.(4)In the co-culturing, combination of Trichoderma viride and other fungus showed higher ability to degrade lignocellulose, illustrated that Trichoderma viride is more suitable to co-culturing.