| Inner Mongolia is a major province of animal husbandry in our country, the sharp contradiction between increasing livestock population and productivity decline of grassland makes livestock going through nutrient restriction.lt will seriously affect animal growth and production performance. Adipose tissue is an important part of the body, the body’s largest endocrine organ. Adipose tissue going through significant changes during growth restriction and compensation. However, the related endorcinology changes is lack of research. This experiment was focused on the changes of adipokine gene expression of subcutaneous and visceral adipose tissue of lamb after feed restriction and compensation. Twenty Mongolian lambs (four months old), were randomly divided to control group (C group, ME 8.95MJ/kg, CP 10.68%) and feed rectricted group (R group, ME 7.84MJ/kg, CP 8.28%). The experiment included two stages:60d of feed restrction and 60d of realimentaion. Lambs were all fed same diet (ME 9.85MJ/kg CP 12.22%) during the compensation stage. At the end of the each stage, five lambs was slaughtered and adipose tissue (femoral, omental and perirenal) were collected. Quantitative real-time PCR were used to determine gene expressions ofperoxisome proliferator activated receptor y (PPARy),Sterol regulatory element-binding protein 1(SREBPl), acetyl coenzyme A carboxylase (ACACA), fatty acid synthase(FASN), perilipin, adiponectin, leptin, tumor necrosis factor (TNFa),interleukin 6(IL-6) andIL-1β. During the experiment, the blood sampled were also collected at 30,60,90 and 120d and the realated parameters (glucose, insulin, triglycerides, cholesteral and non esterified fatty acid) were determined. The experiment results were showed as follow:(1) The body weight of control group was increased from 22.22 kg at the beginning to 33.84 kg at the end. The average daily gain was 0.193 kg/d. The body weight of the R group was constant with little increase during the restriction period. After 60d of compensation, the average daily gain of R group was 0.084 kg/d and 0.077 kg/d higher than C group at 60 to 90 d and 90 to 120 d.(2) After 60 d of feed restriction:PPARy mRNA was significantly decreased in femoral and omental fat, while SREBP was only decreased in femoral fat. ACACA and FASN gene expression was decreased in femoral, omental and perirenal fat. Perilipin(3) mRNA was decreased in all three fat indicated increased mobilization of the fat. Adiponectin was only decreased in omental fat and leptin gene expression was decreased in all three adipose tissue. TNFa mRNA was decreased in femoral fat. IL6 mRNA was decreased in femoral fat, but increased in omental fat. IL-1βmRNA was increased in all three fat depot. After 60d of compensation:SREBP mRNA was increased in omental and perirenal fat, while ACACA was only increased in omental fat. Adiponectin, leptin and TNF was still lower in perirenal fat depot.(4) Plasma TG, glucose and insulin level was reduced by feed restriction. The NEFA concentration was increased at 30d of feed restriction and followed to the control level at 60d. No difference was found in cholesterol level. After 60 day compensation, all parameters were recovered.Feed restriction induced different changes in femoral, omental and perirenal fat depot. The increased expression of inflammatory factors may involved in the regulation of fat metabolim. Present experiment provided fundamental data for the related researches. |
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