The Research Of Rapid Propagation In Vitro And The Factors Of Leaf Color Variation On Epipremnum Aureum

This test mainly selected the explant and the steriling time, and researched the influence of different growth regulator on callus induction, adventitious bud regeneration, multiplication of subculture, control of aerial root, and induced of adventitious root by tissue culturing of Epipremnum aureum and Scindapsus aureus var. wilcoxii. And this test solved the problem of browning at the callus induction and subculture process, finally it established the completed tissue culture and rapid propagation system of Epipremnum aureum. Moreover, this test has studied the variation of the color on leaf in the tissue culturing, which included the major factors for the variation, the separation of this character and expanded propagation the pure variety. The primary test results as follows:1 At the primary culture of Epipremnum aureum, the best explant is stem, and the optimal way of sterilization is secondary sterilization for 8+10min. The best medium for callus induction is MS+TDZ2.0mg/L +2,4-D0.2mg/L. At the multiplication of subculture process, first, the best medium for adventitious bud regeneration is MS+6-BA1.0mg/L+NAA0.3mg/L +TDZ0.2mg/L. Second, the best enrichment culture medium is MS+6-BA1.0mg/L+NAA0.2mg/L, and injecting the PP333 about 0.2mg/L, which growth coefficient can be enhanced obviously. Moreover, injecting 15ml perlite per 50ml solid medium can control the growing of aerial root better. The best medium fot the abduction of adventitious root is 1/2MS+NAA0.2mg/L.2 The rate of browning of Epipremnum aureum is higher than Scindapsus aureus var. wilcoxii, and the rate of browning of stem is higher than stipe and leaf. In the callus induction section, joining 2.0g/L activated carbon or 8.0g/L citric acid and cultivating in darkness condition can control the browning better. In the subculture and enrichment culture section, the rate of browning is more and more higher with the times of subculture increased. Injecting l.Og/L activated carbon before the fourth subculture, 2.0g/L activated carbon before the eighth subculture, and 3.0g/L activated carbon after the fourth subculture can control the browning better.3 The aberration rate of sprouting in callus is higher than sprouting in stem base directly of Epipremnum aureum, and the aberration rate of sprouting in callus and in stem base directly is undifferentiated of Scindapsus aureus var. wilcoxii. In the subculture and enrichment culture section, which hormone species and concentration has significant difference for the aberration rate in subculture process of this two variety., and the influence power for aberration rate is 6-BA>NAA>IBA. With the increase of illumination intensity, the aberration rate of Epipremnum aureum is increased, but the aberration rate is reduced in Scindapsus aureus var. wilcoxii. With the increase of progenitive times, the aberration rate is on the rise, and the aberration rate by tissue culture is lower than cutting propagation of Epipremnum aureum.4 By rejecting the variant plant in subculture process, and then breeding the pure Epipremnum aureum and Scindapsus aureus var. wilcoxii, which result showed that with the increasing of times of subculture, the aberration rate of this two varieties is declined. When it was subcultured six times,the aberration rate kept relatively stable and lower.