Effect Of Yeast Culture On Rumen Fermentation, Nutrient Flow And Digestion In The Gastrointestinal Tract In Sheep

This study aimed to get the suitable addition of yeast cultureby in vitro and in vivo tests,and evaluate the effect of two kinds of yeast culture : XP, YC1 on rumen fermentation and nutrient flow and digestion in the gastrointestinal tract of sheep. Nine sheep with an average weight(35±2.67)kg, fitted with ruminal duodenum and ileum cannulas for test. The paper is divides into three parts as follows:First, The suitable addition of yeast culture was to get under the basal diet with C/R ratio of 3:7 by in vitro and in vivo tests and comparing YC with DiamondV XP. By using two factors cross group design,The test was divided into two phases:The first part was in vitro experiment. The test was divided into five groups( I,II,III,IV,V) based on the concentration of YC(0%、1%、1.5%、2% and t 2% DiamondV XP). Each group had three parallel.The second part was in vivo experiment. The test consisted of three groups inclouding:the control group and the top two leading concentration of yeast culture in vitro test and each group had three parallel.The concentration of pH,NH3-N,and VFA were measured for each sample of in vitro and in vivo in the 3 times.The in vitro results showed that on the whole tend, the effect of 1.5%、2% yeast culture on rumen fermentation was similar to DiamondV XP. Significantly decreased NH3-N concentration, increased acetic acid, propionic acid, butyric acid concentration(P<0.05).And the in vivo results showed that 1.5% group can significantly increased TVFA concentration, decreased NH3-N concentration(P<0.05).1.5% yeast culture was the suitable addition.Second,This study aims to evaluate the effect of two kinds of yeast culture(YC) : XP, YC1 on rumen fermentation of sheep. Treatments were 1) The control group; 2)feed 1.5%YC1; 3)feed 2% XP. The results showed that the effect of group I、group II yeast culture were Significantly increased pH(P<0.05), There was no significant effect of pH in group I and group II. The concentrations of NH3-N in group II were significantly lower than in group I(P<0.05), The concentration of NH3-N in group III was significantly lower than in group I(P<0.01)and group II(P<0.05). group II and III can increased BCP concentration(P>0.05), acetic acid, propionic acid concentration(P<0.01). The results suggest that YC can stabilize rumen pH value and increased BCP(P>0.05)、TVFA concentration(P<0.01), decreased NH3-N concentration(P<0.01).Finally,This study aims to evaluate the effect of two kinds of yeast culture(YC) : XP, YC1 on nutrient flow and digestion in the gastrointestinal tract of sheep. Treatments were 1) The control group; 2)feed 1.5%YC1; 3)feed 2% XP. Both kinds of YC reduces the rumen liquid flow rate, rumen liquid volume and the residence time and rectum digesta DM, OM flows(P<0.05), XP reduces the rectum ADF flow(P<0.05). Both kinds of YC increase ruminal digesta MN and duodenal MN flows and DM, OM, ADF and NDF digestibilities in total tract(P<0.05). YC increases CP digestibility in small intestine(P<0.05). The results suggest that YC is by improving the digestibility of nutrients in the small intestine to improve the total tract digestibility.