| In this paper, the rhizosphere microbial were isolated and identified about Pinus tabulaeformis and Ostryopsis davidiana's different forest types using traditional separation methods, and tested its functional diversity using BIOLOG methods. The results showed that:(1) Rhizosphere soil under different forest conditions used different carbon sources. Especially in the mixed forest, wide range of microorganisms in the soil showed high activity and functional diversity.(2) Diversity among different forest types was different. Microbial diversity were not obvious differ between rhizosphere soil and soil of surrounding root. The numbers of microorganisms were biggest in mix forest's rhizosphere soil, and its distribution was uniform.(3) Microorganisms in mixed forest were the most stable, because its degree of dispersion was the minimum. Studied the correlation matrix PCA, the carbohydrate influenced PC1, followed by carboxylic acid, amino acids, and multi-cluster compounds; The greatest contribution to the PC2 carbon source was amino acids; The greatest contribution to the PC3 carbon source was carbohydrate.(4) Through the form observation 17 genera were identified including 76 strains of fungi; Bacteria were isolated and purified 108 strains, which were identified 84 species of 10 genera; Actinomycetes were identified in 31 strains of 7 genera.(5) Season was the greatest impact to the pH of Pinus tabulaeformis's rhizosphere soil, the minimum impact to the pH of Ostryopsis davidiana's rhizosphere soil.(6) Season was differ effecting the numbers of microorganisms in different forest types.(7) In this paper, analyzed microbial species diversity index, evenness, richness, dominance about different periods and different forest types. The results showed that the microbes are more evenly distributed. Microbial populations of different forest type in September were more stable than in April and July. |
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