| In recent years Dalian Jin Pu District DengShaHe bay waters breeding kelp large-scale decay disease, so that farmers suffered huge economic losses. Waters and environmental survey results, kelp culture areas surrounding water environment in a heavy metal ion in excess of the phenomenon. To study environmental factors and metal copper ion, the effect of nickel on kelp growth and related physiological indexes, in den Shahe and liguhai domain for tracking environmental factors collected kelp samples do a study on the growth.On the other hand, under laboratory conditions, respectively, set of different concentrations of Cu( Ⅱ) and Ni( Ⅱ) cultivation water environment, the concentration gradient for 0,0.25,0.50,1.00, 5.00mg/L. of Laminaria japonica were 7 days of indoor culture experiments. The two kinds of heavy metal ions on the growth of Laminaria japonica situation, adsorption of metal ions, soluble sugar, protein content, total antioxidant capacity, the influence of superoxide dismutase(SOD) activity. The concentration of copper and nickel is set to 0,0.25,0.5, 1.0,5.0mg/L.The results are as follows:(1) Two kinds of effects of metal ions on kelp growth: Under the role of Cu2, seaweed growth was significantly inhibited, appear thinner and even decay and other symptoms, and the higher the concentration, the phenomenon is much more obvious. While in the Ni2 kelp in the experimental group did not appear to be rotting symptoms. Compared with the control group. In the experiment of nickel ion concentration range, each kelp growth rate had no significant difference, and weight gain rate were significant difference. For copper ion effects on the growth of Laminaria japonica, control Laminaria group and four in the experimental group kelp growth rate were significantly different, 1.0 and 5.0 mg / L in the experimental group kelp increasing weight rate with a significant difference in the change between the three groups before.(2) Kelp of two metal ions adsorption: with Cu(Ⅱ), the increase of the concentration of Ni(Ⅱ), kelp for adsorption of both ions are increasing trend, with 1.00 to 5.00mg/l increased significantly and 5.00mg/l concentration is the highest. The comparison between the two adsorption of Cu2 + with kelp is significantly stronger in the adsorption of Ni(Ⅱ).(3) Effect of two kinds of metal ions on the nutritional composition of kelp: Effect of Cu(Ⅱ) of kelp soluble sugar with the concentration increasing decreasing state. When the concentration of 0.25 mg / L decreased significantly, and in the concentration of 1.00 mg / L in the content was the lowest, less than control group. And Ni(Ⅱ) effects on sugar content of kelp and Cu(Ⅱ), showed a trend of first increased and then decreased, at a concentration of 1.00 and 0.50 mg / L increased significantly and 0.50 mg / L reached highest and higher than that of control group.Effect of Cu(Ⅱ) kelp protein is with increasing the concentration of the overall trend was first increased and then decreased, and at a concentration of 0.25 mg / L significantly increased, and reached the highest. In three concentrations decreased significantly, was the overall upward trend in 5.00mg/l is the lowest.Ni(Ⅱ) on Laminaria japonica, but the increase was not significant. And at a concentration of 0.50 mg / L, content decreased, but not significantly.(4) Effect of two kinds of metal ions on the antioxidant activity of kelp:Cu(Ⅱ) and Ni(Ⅱ) kelp total antioxidant capacity of the influence of different trends, in under the action of Cu2 kelp the total antioxidant capacity of performance is decreased at first and then increased, and the change was not significant, at a concentration of 0.25,0.50,1.00mg/L lower than control group, and at a concentration of 0.50 mg / L to the lowest. And under the action of Ni( Ⅱ) kelp total antioxidant capacity performance for increased first and then decreased, at concentrations of 0.25 and 0.50 mg / L when increased most significantly, and in the concentration of 0.25 mg / L reached the highest when the concentration is more than or equal to 0.50 mg / L, kelp total antioxidant capacity began to decline, the concentration of 0.50 mg / L and the control group had significant difference after two concentration group and the control group, the difference was not significant.Effect of Cu(Ⅱ) of Laminaria japonica SOD with the concentration increase showed a trend of decreased first and then increased, drops in the 0.25,0.50mg/L is most remarkable, at concentrations of 0.25 and achieve the lowest when the concentration is more than or equal to 1.00 rise was significantly higher than that of the first two experimental group(the concentration of.Ni2 in 0.25,1.00,5.00 mg / L to the sea with the effect of SOD increased and were not significant, but in concentration is 0.5mg/l effect of Laminaria japonica sod is decreased, and the decreased significantly.(5) The effects of two kinds of metal ions on Photosynthesis and respiration of kelp:Kelp in the experimental group under the effects of Cu(Ⅱ) photosynthesis decreased significantly, when Cu(Ⅱ) concentration of 0.25 and 0.5 mg / L decreased significantly, when the concentration of 1.00 mg / L and 5.00mg/l decreased significantly and attain negative values. And the impact the performance of respiration, increased first and then decreased with increasing concentration, the concentration in the 0.25,0.50,1.0mg/L respiration intensity increased, at concentrations at 1.00 mg / L reached maximum, and increased significantly. When the concentration of 5.00mg/l decreased significantly, and lower than that of the control group.In under the influence of Ni(Ⅱ) kelp photosynthesis and respiration photosynthesis trend is to rise first and then decline, when Ni(Ⅱ) concentration was 0.25 mg / L increased significantly and reached highest at 0.50 mg / L increased, but not significantly, in the greater than or equal to 1.00 when began to decline, but not significantly, when the concentration reached 5.00mg/l significantly decreased. Effects on respiration is shown as at a concentration of 0.25 mg / L significantly increased, and reached the highest at other concentrations did not change significantly.(6) Investigation of environmental factors in the sea area of Shahe and its influence on the growth of kelp:Shahe sea water temperature, PH, the average value of salinity is lower than that of Dalijia area, average value of dissolved oxygen and transparency is higher than that of Dalijia waters. On the other hand, the changes of each index, a number of environmental factors on fluctuations in Shahe sea area is larger, and Da Li this domain is relatively stable two. From the sea kelp growth situation, the overall characteristics of Shahe sea kelp is partial length and width, and the growth characteristics of this domain is the emphasis on Li kelp, in relative growth, Da Li’s sea kelp growth is better than climbing the Shahe sea, kelp, stable growth, and reached Shahe sea kelp may be due to environmental factors due to the unstable sea kelp growth also have the characteristics of volatility, the volatility generally occurs after fourth or fifth times of sampling is from winter into spring, Climate change in this period is complex, may be caused by such instability fluctuations in the cause of this instability may cause fluctuations in the waters of kelp appeared massive disease. |
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