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Comparative Study On Blood Biochemical Indexes Between Healthy And Ailing Green Turtles (Chelonia Mydas)

Posted on:2017-05-06Degree:MasterType:Thesis
Country:ChinaCandidate:Q ChengFull Text:PDF
GTID:2283330509456158Subject:Clinical Veterinary Medicine
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Firstly the green sea turtle blood learn research status review, summarize the domestic and foreign green turtle breeding and wild green turtles hematology research, summarized the progress of green turtle blood cell research status and blood biochemical studies. Combined with the common physiological and pathological changes of green turtles, the effects of diseases and physiological activities on blood biochemistry of green turtles were reviewed. Detection of blood biochemical indicators is an important way to reflect the physiological and pathological changes of the organs and organs, through which the physiological and pathological state of the various organs of the organism can be reacted. Due to the domestic green turtle breeding stay in the appearance of observation and experience, can not deal with all the problems occurred in the course of breeding. In clinical practice, did not have one brought forward the method of scientific and effective diagnosis of illness, and because the blood examination in biology examination has high accuracy, fast diagnostic speed, so this paper summarizes finishing the research on green turtle blood. As the research develop green turtle blood fluid index standard reference.The green sea turtle blood biochemical as the starting point and the foothold, through the acquisition of Shanghai Ocean University climbing World Aquarium Pet Health Center from hatchling green sea turtle venous blood biochemical detection, artificial breeding healthy larvae of green turtle heart, liver and kidney of blood biochemical indexes of reference range is proposed for the first time. Study found that wild individuals of biochemical indexes and artificial breeding of green turtles differences larger, aspartate aminotransferase(P=0.002), albumin(P=0.015), alkaline phosphatase(P=0.010), glutamic pyruvic transaminase(P=0.010) etc. were significantly different, artificial breeding green turtles except for alkaline phosphatase and age showed a negative correlation, there is no significant difference, individuals of different sex in biochemical indicators had no significant difference.In this paper, in 15℃, 20℃ and 25℃ and 30℃ temperature of cultivation of larvae of green turtles 60 days, the temperature study of green turtle heart, liver and kidney and blood biochemical indicators of 17 influence. The results showed that the heart function index, total cholesterol, triglyceride and low density lipoprotein protein increased firstly and then decreased trend, the maximum concentration at 25 DEG, respectively 3.83±0.30 mmol/L, 0.08 mmol/L, 0.60 + 2.08 + 0.10 mmol/L, high density lipoprotein changes rose after the first drop, creatine kinase the concentration increased with temperature and decreased; the index of liver function, albumin and total protein concentration with the temperature rise, increased after the first drop, reached a maximum of 6.47 + 0.66 + 1.96 g/L and 36.33 g/L at 25 DEG C, lactate dehydrogenase, alkaline phosphatase, aspartate aminotransferase, alanine aminotransferase and total bilirubin were with the change of water temperature changes(P>0.05); renal function index, serum creatinine and uric acid concentrations decreased with increased temperature, urea nitrogen without significant changes(P>0.05). The effect of temperature on blood biochemical index was studied, and the energy storage material consumption of green turtles at about 25 degrees C was the least. 25 degrees Celsius can be used as the most suitable growth temperature of green turtles, to facilitate the management of conservation.As a large marine reptiles, green sea turtle body most is a Phi attached, in the course of feeding, to avoid being infected with the parasite, with the passage of time caused ulceration skin abscess, and even endanger the life of hatchlings. Green sea turtle blood biochemical indexes of this chapter through the spin nematode infection found that green turtles are infected blood biochemical indexes except for aspartate aminotransferase did not occur significant change(P>0.05), but from at home and abroad is serious in reptiles speculated that turtles after ingestion of spin of nematode eggs or larvae, larvae after the circulation of the blood reaches the liver, cause(compared to the non infected group increased by 38%) elevated alanine aminotransferase. In addition, eosinophils were significantly higher in the non infected group(P<0.05).In this paper, the changes of blood biochemical indexes, physiological and pathological changes of green turtles were studied from the aspects of artificial breeding environment temperature, common parasite and health assessment. To lay the foundation for the study of the blood and physiology and pathology of green turtles in the future, and to establish a reliable basis for clinical diagnosis and treatment.
Keywords/Search Tags:green turtle, blood biochemistry, spirurid worm
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