Isolation And Purification Of Chemical Composition From Polygala Tenuifolia Wild And Dallbergia Odorifera

Chinese traditional medicine herbs are abundant. Preparation of high purity activecomponents is important to further study, such as exploration of pharmacology andeffective control of quality.For the time being，new methods of separation，purification and detection ofPolygala tenuifolia Willd and Dalbergia odorifera have been built. The detectedconditions were optimized at the same time to detect the extracts and the monomer.PHPLC and different column chromatography were used for the separation andpurification of the targets. The proportion, flow rate and the volume of injection wereoptimized as well. The structures were identified by UV and NMR ultimately1. Isolation and purification methods of flavonoids from Polygala tenuifolia Willd.An opportune extract metod was established：extraction solvent was methanol andwate（r70：30v/v）, extraction method was heated to reflux for2hours by3times.,and theseparated the crude extracts roughly based on macroporous resins. Active ingredients ofPolygala tenuifolia Willd were divided into three parts approximately.（1）Isolation and purification methods of TenuifolisdeA，Tenuifolisde BThe first part was further isolated and purificated by ODS.Tenuifolisde A andTenuifolisde B were obtained with a high purity. The purities were92.3%91.2%respectively.（2）Isolation and purification methods of six sugar esters from Polygala tenuifoliaWilldThe method of separation and purification of sugar esters from Polygala tenuifoliaWilld has been established which combines PHPLC with ODS column chromatog-raphy.The best separation result was obtained when methanol and water（50：50v/v）used as mobile phase in gradient elutin mode; the flow rate of the mobile phase was20mL/min,the injection volume was1.0mL the detection wavelength was330nm duringthe separation procedure. Six sugar esters were prepared from Polygala，tenuifolia Willd.The purities were93.1%，92.8%，98.7%，99.0%，90.1%，90.2%respectively. （3）Isolation and purification methods of flavonoid and other kinds of compoundsfrom Polygala tenuifolia WilldThis experiment was also to establish a new method of the separation andpurification of flavonoid and other kinds of compounds from Polygala tenuifoliaWilld.A PHPLC method was developed for separation and purification with GLP-ID(400mm×25.4mm);the best separation result was obtained when methanol and water（43:57v/v）were used as mobile phase in gradient elution mode; the flow rate of themobile phase was20mL/min,the injection volume was2.0mL,and the detectionwavelength was330nm during the separation procedure. A cinnamic acid, a fructoseand a glycosides were purified at last. The purities were95.3%,90.8%,94.2%respectively.2. Isolation and purification methods of flavonoids from Dalbergia odoriferaIt was also to establish a method for the separation and purification of flavonoidsfrom Dalbergia odorifera. The optimizsd extract method was that: extraction solvent wasethanol and water（70：30v/v）, extraction method was heated to reflux for2hours by3times. The rude extracts were separated rudimentary by silica gel columnchromatography. Optimized conditions were that: silica gel column（prepared byourselves，the detection wavelength was275nm during the separation procedure,, themobile was ethyl acetate-petroleum ether system in gradient elution。Six activecompounds were separated successfully, their purities were97.8%,98.6%,98.5%,97.9%,98.2%,98.1%respectively. The structures of the components were identified by UV andNMR.