Analysis Of Chinese Agarwood By X-ray Diffraction Spectroscopy,Infrared Spectroscopy And Ultraviolet Spectroscopy

Chinese agarwood is a containing resin timber of Thymelaeaceae plant Aquilaria sinensis distributed in Hainan, Guangdong, Guangxi, Yunnan, Hong Kong, and Taiwan provinces, etc. As a traditional Chinese medicine, agarwood which is usually used as Chest pain, bloating, nausea, vomiting, hiccups stomach, kidney gas pain, etc. In this paper, analysis and comparison of the quality of8Chinese agarwoods were carried out by3modern analytical techniques including X-ray diffraction spectroscopy, Infrared spectroscopy and Ultraviolet spectroscopy. In addition, allelopathic effects of agarwood were studied.First, results of X-ray diffraction (XRD) analysis of the Chinese agarwood showed that the similarity between8batches of agarwood samples were0.9096-0.9601, and results of clustering analysis were consistent with that of similarity analysis. Clustering and similarity analysis of wild and artificial agarwood samples demonstrated that the similarity of Chonglou agarwood and Laotou agarwood are highest (0.9900) and were classified as a group, and that of huangyou agarwood and Diaokou are higher (0.9600) and were classified as a group. Similarity of Niujiaoer, Holing and Gousi are lower (0.9000) as a class, and that of Baotou, Laoding and agarwood produced by infusion are the lowest(0.8700). Similarity and clustering analysis of Chinese agarwood from different places indicated that the similarity of agarwood from Hainan is highest (0.9900), that from Yunnan is higher (0.9600), and those from Guangdong and Guangxi are lower (0.8700).Secondly, results of Infrared spectroscopy (IR) and Ultraviolet spectroscopy (UV) showed that8batches of Chinese agarwood samples are more consistent, indicating its good stability. Wild and artificial agarwood samples are more consistent with XRD analysis. Comparison of agarwood from different places by IR and UV demonstrated that agarwood from Hainan and Yunnan are more similar, and those from Guangdong and Guangxi are more similar.Finally, The allelopathy of agarwood showed that volatiles of agarwood stimulates seedling growth and seed germination of the four vegetable (Cucumis sativus L., Brassica pekinensi, Raphanus sativus L., Brassica campestris). Low concentration of the water extract of A. sinensisis can promote the seeding growth of the four vegetable, while high concentration suppress it.Infrared spectroscopy (IR), Ultraviolet spectroscopy (UV) and X-ray diffraction (XRD) have advantages such as simple sample preparation, high measurement speed, good repeatability, etc. Combining the methods above can be applied for the identification of agarwood effectively and accurately. Simultaneously, the methods above provide scientific means for the quality evaluation of agarwood. Study on the allelopathic effects of agarwood and A. sinensisis on crops provides basis for the inter-plantation of crops in the forest of A. sinensisis.