| Objective:To investigate the relationship between the expressions of AngⅡ/ATIR and the abilities of proliferation, migration and invasion in human bladder carcinoma cell lines. To compare the differences of proliferation, migration and invasion between three bladder cacinoma cell lines, to explore the effects of proliferation, migration and invasion by AngⅡ in these cells, detecting the expressions of ATIR in different bladder cacinoma cell lines, to find out new experimental data and potantion target in the treatment of bladder cacinoma.Methods:The expressions of AT1R mRNA in three bladder cacinoma cell lines were determined at the level of mRNA by Real-time quantiative Polymerase Chain Reation(qRT-PCR). The expressions of AngⅡand AT1R protein in three bladder cacinoma cell lines were determined at the level of protein by Western Blot. Detecting the proliferative abilities between different bladder cacinoma cell lines, to observe the influences of cell proliferative ability after using Ang Ⅱ by MTT method. Detecting the migratory abilities between three cell lines, to observe the influences of cell migratory ability after using Ang Ⅱ by scratch test. To detect the invasion abilities between three cell lines, relationship was analyzed between invasion and the action of Ang Ⅱ by cellular invasion assays,Results:The mRNA of ATIR was expressed in three cell lines, the relative optical density wasEJ-M3(15.43±0.5533), EJ(4.23±0.5951), BIU-87(1), the differences were significant between three cell lines(p<0.01). The protein of AngⅡ don’t expressed, but AT1R expressed in three cell lines, the relative optical density was EJ-M3(0.6891±0.1264), EJ (0.5198±0.1223), BIU-87(0.0252±0.0112), the differences were significant between three cell lines(p<0.05). The absorption value at10-4mol/L、10-5mol/L、10-6mol/L AngⅡ of EJ-M3experimental group(0.6096±0.02334,0.6089±0.03513,0.6115±0.0248) was indifference than the control group(0.615±0.0284,0.6134±0.02788,0.6155±0.02244)(p>0.05), The absorption value of EJ experimental group(0.5219±0.01962,0.5211±0.02105,0.5231±0.02197) was indifference than the control group(0.5266±0.01798,0.524±0.01922,0.5184±0.02285)(p>0.05), The absorption value of BIU-87experimental group(0.4998±0.00793,0.4964±0.01156,0.4971±0.01335) was indifference than the control group(0.5009±0.00893,0.4794±0.01247,0.4968±0.01247)(p>0.05). The number of cell migration to the scratch area at12h,24h and36h of EJ-M3experimental group (19.2±1.54919,57.2±3.48967,133.6±4.14193) was greater than the control group (10.1±1.3703,47±2.10819,122.7±4.1379)(p<0.05), the number of EJ experimental group (16.2±1.4757,52.7±3.093,110.1±3.90014) was greater than the control group (8.3±0.94868,39.3±2.66875,97.7±3.49763)(p<0.05), the number of BIU-87experimental group (13.1±2.13177,48.6±2.633,96.5±3.77859) was greater than the control group (7.7±1.25167,37.1±2.64365,78.8±2.39444)(p<0.05), there were significantly differences among three cell lines(p<0.05); The penetrating cell number of EJ-M3experimental group (119.5±3.71932) was greater than the control group (66.2±3.35989)(p<0.01), the number of EJ experimental group (55.2±2.93616) was greater than the control group (33.5±3.24037)(p<0.01), the number of BIU-87experimental group (37.6±3.30656) was greater than the control group (20±1.8574)(p<0.01), there were significantly differences among three cell lines(p<0.01).Conclusion:The biological characteristics of three bladder cacinoma cells were different, it was found that the abilities of proliferation, migration and invasion in three bladder cacinoma cells were different, the abilities of migration and invasion in bladder carcinoma cells were promoted by using Ang Ⅱ, but did not effer the growth of three cell lines. The expression of AT1R was positively correlated with cells’ proliferation,migration and invasion capability, Ang Ⅱ/AT1R axis plays an important role in the invasion and metasitasis of bladder cacinoma,it will become a new potention target to treat bladder cacinoma. |
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