The Influence Of TAC1and Efflux Pump Genes On Biofilm Formation In Candida Aibicans

[Objective] To explore the role of resistance-related genes TAC1MDR1CDR1CDR2in the process of biofilm formation of Candida albicans.[Methods] Biofilms were formed and the susceptibility to fluconazole in different biofilm periods of C. albicans was respectively determined by MTT method.The total RNA was extracted from biofilms in different phase and then cDNA was synthesized.The expression of biofilm-related genes was detected by quantitative real-time PCR.[Results] ANOVA test(p<0.05) indicated that the expression of TAC1MDR1CDR1and CDR2had meaningful differences in the process of candidal biofilm formation. Bonferroni test (p<0.05) revealed that the expression of TAC1MDR1CDR1and CDR2in the earlier phase was higher than that in the middle and late phases for the isolate00889.For the clinical isolate359,the expression of TAC1MDR1CDR1and CDR2in the middle phease was higer than that in the late phase.[Conclusion] It is likely that the TAC1and efflux pump genes may play a role in the resistance to fluconazole of candidal biofilms in the early and middle phease. Multiple molecular mechanisms lead to the resistance to azoles in C. albicans, so in the future further explore should be done to beter understand molecular mechanisms of resistance to azoles.