The Proportion And Clinical Significant Of Myeloid Derived Suppressor Cells In Peripheral Blood And Tumor Foci Of Gastric Cancer Patients

Objective: To investigate the frequency and clinical significance of Myeloid-derivedsuppressor cells in peripheral blood and tumor foci of the patients with gastric cancerMethods:1．The peripheral blood specimens of36gastric cancer patients and10healthy adults were collected in the Department of General Surgery of the First Affiliatedof Soochow University. Make the expression pattern of peripheral blood white cells(PBWC) in HLA-DR~-CD14~-CD33~+as monocyte derived MDSC. Flow cytometry was usedto detect and analyze the frequency and clinical significance of MDSC in peripheral bloodof two groups.2．The tumor foci(tumor nest site) specimens which was operationed excision of36gastric cancer patients were collected. Flow cytometry was used to detect and analyze theexpression pattern of tumor foci in HLA-DR~-CD14~-CD33~+MDSC in tumor cases;3．Analyse the relationship between the biochemical indicators of patients with gastriccancer patients and the proportion of HLA-DR~-CD14~-CD33~+MDSC.4．Use statistical software to analysis the influence of HLA-DR~-CD14~-CD33~+MDSCin tumor progression and prognosis, combining with clinical data such as age, gender,tumor size, lymph node, metastasis, distant metastasis, histologic type, degree ofdifferentiation and TNM stages.5．Collect the peripheral blood specimens(n=15) of gastric cancer patients at two timepoints(before postoperative and30days after operation),according to the above researchprogram and analysis the dynamic relationships between the proportion ofHLA-DR~-CD14~-CD33~+MDSC and tumor load.Results:1．The proportion of HLA-DR~-CD14~-CD33~+MDSC in the peripheral bloodof gastric cancer patients is significantly higher than the healthy humans’. 2．The proportion of HLA-DR~-CD14~-CD33~+MDSC in the peripheral blood ofgastric cancer patients is significantly higher than the proportion of HLA-DR~-CD14~-CD33~+MDSC in The tumor foci(tumor nest site).They have positive relationship with theproportion of HLA-DR~-CD14~-CD33~+MDSC.3．The proportion of HLA-DR~-CD14~-CD33~+MDSC in the peripheral blood ofgastric cancer patients has negative relationship with ALB, GGT, TT, and positiverelationship with CRS.4．The proportion of T3and T4infiltration degree gastric cancer patients is higherthan the proportion of MDSC of T1and T2infiltration degree patients, the proportion ofMDSC with distant metastases is significantly higher than those without distant metastases,gastric cancer patients with lymph-node metastasis express much more MDSC than not,the proportion of MDSC of Ⅲ and Ⅳ period gastric cancer patients is significantly higherthan the proportion of Ⅰand Ⅱperiod patients.5．The proportion of MDSC of gastric cancer patients decreased obviously at the30thday after operation, which is significantly higher than before the operation.Conclusion:1．The proportion of HLA-DR~-CD14~-CD33~+MDSC in peripheral bloodof gastric cancer patients is related to the occurrence and progression of tumor.2．The proportion of MDSC decreases after resection, which suggests that the immunefunction recovered in a certain extent.3．The proportion of MDSC in peripheral blood may become a good cellularimmunological index of cancer patients in clinical monitor.4．MDSC provides a new way of gastric cancer immunotherapy.