| Objective To detect the expression changes and distribution of HGF(Hepatocyte growth factor) and its receptor c-Met (Hepatocyte growth factorreceptor) in liver regeneration after hepatic blood flow blocking and hepatectomy inrats and to investigate the role of HGF/c-Met in the regulation of liver regeneration.Methods Forty healthy SD rats were randomly divided into two groups, five rats inthe normal control group and thirty-five rats in the model groups. The rats in thenormal control group were put to death and taken normal liver tissue withouthepatic blood flow blocking. The model group rats were performed hepatectomy afterhepatic pedicle trigeminy block as long as twenty minutes and were taken the residualliver tissue after3h,6h,1d,2d,3d,5d and7d. The materials of the normal controlgroup and model group in each period of time were histologically processedand stained with HE. Morphological changes of liver tissue were observed undermicroscope. The expression changes and distribution of HGF and c-Met at eachperiod in liver regeneration after hepatic ischemia were examined by immuneorganized PV9001rabbit allergic two steps.Results Compared with normal liver tissue, the obvious inflammatory reaction inliver tissue of the model group rats was visible in the early liver regeneration,characterized by liver cell edema, cytoplasmic loose change and part of the balloonsamples. Meanwhile, dual-core liver cells and dot necrosis were visible. A largenumber of neutrophils infiltrated, and then the inflammatory response was relievedafter1d. A small amount of expression of HGF appeared in the liver tissue of thenormal control group rats. Expression were mainly distributed in the liver cell cytoplasm. The expression of HGF continued to increase in the postoperative residualliver tissue of the model group rats, increased slowly from3h to1d, rapidly from1dto3d and then tended to be stable from3d to7d, but compared with the normalcontrol group rats, the high expression appeared at7d. There was also a small amountof expression of c-Met in the liver tissue of the normal control group rats. Expressionwere mainly distributed in the liver cell cytoplasm and cell membrane. The expressionof c-Met decreased in the postoperative residual liver tissue of the model group ratsfrom3h to2d, decreased to the Minimum at2d, but increased gradually from2d to7d, and was closed to the expression level of the normal control group. Comparingthe model group with the control group, the expression differences of HGF and c-Methave statistical significance (p<0.05).Conclusion The increased expression of HGF is induced by hepatectomy, and theexpression of c-Met decrease firstly and then increase. HGF/c-Met may be one of theimportant factors of the molecular regulation of liver regeneration. Because of theischemia reperfusion injury, the expression of HGF increase slowly in the earlypostoperative liver regeneration. The ischemia reperfusion injury may be an importantfactor which reduce liver regeneration level and delay liver regeneration process. |
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