Expression And Significance Of INPP4B And PTEN Protein In Hepatocellular Carcinoma

BackgroundPrimary liver cancer is one of the most common malignant tumor in clinical,according to the cell classification type,it can be divided into the hepatocellularcarcinoma, bile duct cell carcinoma and hybrid liver carcinoma. HCC (hepatocellularcarcinoma, HCC) is the most common type, its mortality rate is second only to lungcancer, which cause serious damage to human life and health.Traditional treatmentmethod is surgical resection for HCC, but not all patients is suitable for surgicaltreatment. HCC early symptoms are not obvious, so difficult to found. HCC patientsgenerally lost opportunities of operation because cancer cells to spread.Interventional therapy, radiation therapy and targeted drug treatment, the livertransplantation is the main method of treatment of such patients.However, due to theinvasive malignant tumor, even with surgical resection or comprehensive treatment,patients five-year survival rate is still low, the tumor recurrence rate is high.So earlydetection, early diagnosis and early treatment is especially important.In recent years, new molecular targeted drugs in clinical practice has obtainedthe remarkable effect in clinical practice, Such as Sorafenib, Imatinib and Cetuximab.The practice has showed the correctness and feasibility of the molecular targetedtherapy theory. Seeking for a wide variety of tumor target genes or target proteinhas a great role in promoting development of molecular targeted drugs. Phosphatase and tensin homolog does on chromosome ten, PTEN, is a tumor suppressor genelocated in chromosome10(10q23).Research shows inactivation or abnormalexpression of PTEN gene may be plays a very important role in the occurrence anddevelopment of HCC and intrahepatic metastasis biology behavior. Inositolpolyphosphate4-phosphatase type II, INPP4B, is a newly discovered and potentialtumor suppressor, consistent with PTEN function mechanism, through inhibitingPI3K/AKT signaling pathways, can increase cell growth, proliferation, apoptosis, andinduce the apoptosis of tumor cells, thus inhibiting tumor cell invasion and metastasis.However, the function of PTEN and INPP4B and its significance in tumor remains tobe further exploration, there is also little research about its expression andrelationship in HCC in the domestic and overseas.AimTo investigate the expression of INPP4B and PTEN in hepatocellular carcinoma(HCC)and to analyze the level of expression correlated with clinicopathological statusof the patients.To explore its significance in HCC development. To providetheoretical support for the development of the molecular targeted therapy drugs.Methods1．By immunohistochemistry, the expression of INPP4B and PTEN weredetected in74cases of HCC tissue,74cases of adjacent atypical hyperplasia and30cases of normal tissue of same patients;2．Statistical analysis: All the data were analyzed by SPSS17.0statisticalpackage. The Chi-square was used in the comparison of positive rates; The relation oftwo variables was analyzed by the spearman level correlation analysis. The level ofsignificance difference was ɑ=0.05.Results1．The levels of INPP4B and PTEN expression in HCC were significantly lower than that in histologically normal hepatic tissue (both P<0.05).2．Both INPP4B and PTEN protein expression in HCC were not statisticallycorrelated with age, sex, cirrhosis background. they were significantly correlated withthe degree of differentiation, tumor capsule. INPP4B protein expression wascorrelated with the tumor size, PTEN protein was not.3．There was positive correlation between INPP4B protein expression and PTENprotein expression in HCC (r=0.561, P=0.000).Conclusions1．NPP4B and PTEN were closely related to the occurrence and development,invasion and metastasis of HCC.2．There was correlation and synergy of INPP4B and PTEN in the developmentof HCC.