Association Of Aldose Reductase C-106T Polymorphism And The Susceptibility Of Essential Hypertension In Chinese Han

Objective:Aldose Reductase (AR/ALR2) is a member of NADPH-dependent aldo-keto reductase superfamily, which is widely expressed in human body in a form of monomer. The AR C-106T polymorphism is located in the basal promoter region of AR gene. The polymorphism of AR C-106T may affect the transcriptional level of AR by changing the efficiency of nuclear transcription factors banding to the CCAAT element. Moreover, the polymorphism of AR C-106T was closely related to the diabetic complications, such as diabetic nephropathy, diabetic retinopathy and diabetic retinopathy. Meanwhile, there were over-expression of AR in myocardium ischemia and vascular inflammation which are characterized by oxidative stress. Aldose reductase inhibitor could ameliorate the myocardium and vascular remodeling in spontaneously hypertensive rats. In previous study, we conducted a preliminary study on the AR distribution in the essential hypertension patients and normal people. Because of the small sample size and low power value, there were no statistical significances between the EH patients and controls. Thus, we have enlarged the sample size to investigate the association between AR C-106T polymorphism and risk for essential hypertension.To investigate the association of AR C-106T polymorphism and the susceptibility of essential hypertension.Methods:AR C-106T polymorphism was genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and the dideoxy chain-termination method. We determined the blood glucose, the levels of triglycerides, total cholesterol, high density lipoprotein and low density lipoprotein. Statistical analyses were performed using SPSS18.0and PASS11. Risk factors of essential hypertension were studied by non-conditional logistic regression analysis and the95%confidence interval and OR were used to evaluate the correlation. The Chi-square test was used to compare the differences on distribution frequency of genotypes and Hardy-Weinberg balance. The differences of sex, family history, age, body mass index, glucose, triglycerides, total cholesterol, high density lipoprotein, low density lipoprotein, systolic blood pressure and diastolic blood pressure between two groups, and the differences of systolic blood pressure and diastolic blood pressure between the AR genotype of CC and (CT+TT) were compared with ANOVA.Results:383Chinese Han healthy volunteers and383essential hypertensive patients were involved in this study. The distributions of all the alleles in EH patients and controls met the Hardy-Weinberg equilibrium. The genotype of AR (P<0.001) and body mass index (P <0.001), family history (P<0.001) were all closely related to the hypertensive susceptibility. Compared with CC genotype,(CT+TT) genotype was the risk factor for essential hypertension (OR=2.68,95%CI:1.780-4.035). The greater body mass index, the greater risk of essential hypertension. Compared with no family history, a person with family history is much easier to get hypertension. We observed that the AR-106T allele frequency was17.5%in EH patients and9.0%in controls, respectively (P<0.001). There were difference systolic blood pressure and diastolic blood pressure between CC genotype and (CT+TT) genotype of AR.Conclusion:The AR C-106T polymorphism was related to the susceptibility of essential hypertension in Chinese Han, AR-106T allele gene might be the susceptible gene of essential hypertension.