| With the arrival of China’s aging population, the incidence of osteoporosis also willincrease, and more and more patients with osteoporosis economic impact on the communitywill be further increased. Currently, there are many drugs about the treatment of osteoporosissuch as estrogen, calcitonin, bisphosphonates, but long-term using these drugs has some sideeffects on people’s health. In the rapid development of science and technology today, with thedanger of osteoporosis gradually perceived by people, coupled with the high price of drugs andthe inevitable side effects, experts have been trying to discover a new treatment for osteoporosis.The emergence of low frequency electromagnetic method brings an opportunity for the majorityof scholars. The method of low frequency electromagnetic fields has no trauma, infection,simple treatment, side effects and other characteristics of osteoporosis in increasing people’sinterest and attention in recent years. As a treatment for osteoporosis physical therapy, thelow-frequency electromagnetic fields is a constantly research for majority of scholars, and theresults are obvious. There are many reports about low frequency sinusoidal alternatingelectromagnetic field with mature osteoblast mineralization, but animal studies have not yetbeen reported.To further understand the role of the low-frequency sinusoidal alternating electromagneticfield on the prevention of osteoporosis and to institute effective electromagnetic parameterscombination of pre-screened based on cell experiments, we have carried out a sinusoidalalternating electromagnetic field research in animal experiments.6weeks of age SPF SD ratswere chosen in these two experiments. Firstly, they were divided into3groups, control,0.1mTSEMFs and0.6mT SEMFs group. Secondly,6-week-old SPF50SD rats were divided intocontrol,45min,90min,180min and270min group. After8weeks, all rats were detected bonemineral density by dual-energy X-ray absorptiometry. Assay of serum bone metabolism relatedfactors were detected with ELISA, detecting biomechanical properties by AG-X series desktopelectronic universal testing machine,and related indicators were detected by VG staining,double fluorescent labeling and animal micro-CT Determination histomorphometry system.From the rat body weight, organ pathology, organ coefficient statistics, we have done a pathological studies, elaborated sinusoidal alternating electromagnetic field trial to improvepeak bone mass and thus prevention of osteoporosis in rats mechanism of action, provide ascientific basis and theoretical basis for research and clinical development of electromagnetictherapeutic apparatus osteoporosis.Our results were what follows next:1. A different field strength screening results showed that50Hz0.1mT group whose BMD,femur and vertebrae bone mineral density and biomechanical properties of bonehistomorphometry related indicators and serum bone formation related indicators significantlybetter than the control group.2. Average of50Hz0.6mT parameter combination of SD rats and its indicators are higher,but not statistically significant.3. Different time screening results showed that sine alternating electromagneticinterference90min of SD rats and its indicators were significantly better than the control group,and180min and270min were significantly better than the control group,but45min averageshigher without significant difference.4. Comprehensive experimental results show that the combination of parameters50Hz0.1mT90min for the sine of the best preventive treatment of osteoporosis alternatingelectromagnetic field effects parameters. Above all,50Hz0.1mT90min sinusoidal alternatingelectromagnetic field can most effectively improve peak bone mass in rats. |
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