The Study Of Effect And Mechanism Of Sihuang Hemorrhoids Ointment Act On Similar Hemorrhoid Model Of Rat

Objective: To investigate Sihuang Hemorrhoids Ointment on similar hemorrhoid model of rat of local symptoms and histopathological changes and on nitric oxide synthase, vascular endothelial growth factor, macrophages changes, and to explore Yin Zhi anorectal smoked lotion on the impact of experimental rats and its mechanism. Yin Zhi anorectal smoked lotion in the clinical treatment of hemorrhoids and other anorectal diseases provide a theoretical basis. Methods: To make similar hemorrhoid animal model use peripheral injection of acetic acid method. the local symptom score animal model to determine the success of modeling and routine pathological examination to determine whether the pathological changes of acute inflammation. Rats after model to be continuous administration of 7 days. After administration of 2,4,7 days observed in rats after administration of general, local swelling, inflammatory exudate and the ulcer area, to estimate the effect of the anti-inflammatory drugs, and promoting ulcer healing, and compare with the control group and Ma Ying-Long Jin Xuan hemorrhoid washing powder control group. On the 8th day after observing the animals were sacrificed by histopathological examination and immunohistochemical examination, observation of drugs on animal models of pathological changes to improve the situation and on i NOS, CD68, VEGF influence, and with the control group and Ma Ying-Long Jin Xuan hemorrhoid washing powder control group for comparison. Results: The animal model of local symptoms scores showed similar rat model of successful modeling hemorrhoids, its pathological examination of pathological changes consistent with acute inflammation. After the administration of Sihuang Hemorrhoids Ointment group to improve local symptoms superior to the matrix group and Ma Ying Long Hemorrhoids Ointment control group(P <0.05 or 0.01); Sihuang Hemorrhoids Ointment group inhibiting i NOS expression were superior matrix group and Ma Ying Long Hemorrhoids Ointment in the control group(P <0.05 or 0.01); Sihuang Hemorrhoids Ointment group inhibition of CD68 expression than the matrix group(P <0.05), Sihuang Hemorrhoids Ointment group and Ma Ying Long Hemorrhoids Ointment group inhibition of CD68 expression is not comparable(P > 0.05). Sihuang Hemorrhoids Ointment group inhibit VEGF expression and the role of the group was superior to the matrix Ma Ying Long Hemorrhoids Ointment in the control group(P <0.05 or 0.01). Conclusion: Sihuang Hemorrhoids Ointment can significantly improve the similar animal model of local symptoms; It has anti-inflammatory and promote ulcer healing; It has a fuction of improving pathological changes of the animal models; It can inhibit the animal model of i NOS, CD68, VEGF expression, the treatment of hemorrhoids of Yin Zhi anorectal smoked lotion by inhibiting i NOS, VEGF, CD68 related.