Study On Chemical Constituents From Actinidia Chinensis Roots And Their Biological Activities

Actinidia Lindl is about 55 specices in the word and exists in the areas fromMalaysia to Russian.Most of the species are mainly exist in China and distributes in the provinces such as Shaanxi, Henan, Hubei and Guangxi. Roots of the Actinidia Lindl are important traditional Chinese medicinal materials in the folk which are used in the treatment of cancer in the digestive system, jaundice, cacochylia and diarrhea. Roots of Actinidia Chinensis collected in Mei County, Shaanxi are selected in this research.The compounds purified from the roots of Actinidia Chinensis and their anticancer activity were researched.Preliminary study on isolation, purification and biolocial activities of the polysaccharides from Actinidia Chinensis were also conducted.The main results of this research are as folllows:1.Study on the Chemical constituents of the Actinidia Chinensis roots.2 compounds were isolated by chromatography, preparative thin-layer chromatography and recrystallization.The compounds were identified as I(β-Sitosterol),Ⅱ(Ursolic Acid)on the basic of the1 H NMR、13C NMR and ESI-MS.2. Anticancer activity research of the compounds.Anticancer activity on SGC-7901 and A549 cell lines of the 2 compounds were tested.Only compound Ⅱ(Ursolic Acid) showed anticancer activity on SGC-7901 and A549 cancer cell lines after treament of 48 h at the concentration of 50μg/mL with the inhibition ratio of 74.8% and 90.2%,respectively.3. Extraction, isolation and purification of polysaccharides. The defatted roots powder was extracted with distilled water to get the crude polysaccharides.The crude polysaccharides was deproteinated by the method of sevage, before being decolored by5% H2O2 and then was isolated repeatly by DEAE-cellulose and Sephacryl S300 columns.Two homogenous polysaccharides,ACPS1 and ACPS2 with molecular weights of 5.58 × 105 Da and 1.23 × 106 Da were purified,respectively.4. Characteration on ACPS1 and ACPS2.Characteristic absorption peaks of the polysaccharides were showed in FT-IR spectroscopy. The two polysaccharides mainly contained neutral carbohydrate, uronic acid and no protein.SEM images showed that surfaces of ACPS1 and ACPS2 have little difference. GC analysis was used to determine the natural monosaccharide. ACPS1 was mainly composed of rhamnose, arabinose, xylose, mannose and galactose in an approximate molar ratio of 1.48:4.28:4.30:1.00:17.83. ACPS2 mainly contained rhamnose, arabinose and galactose with the molar ratio of 1.00:2.33:6.61.5. Biological activities of the purified polysaccharides. Both ACPS1 and ACPS2 exhibited the remarkable antioxidant activity to scavenge the DPPH radical and significant protective effects on H2O2-induced HEK 293 cells death. Meanwhile, in vitro immunomodulatory activities of the two polysaccharides were evaluated. The results showed that ACPS1 and ACPS2 have no cytotoxicity on Raw264.7 and treatment with 50–300 μg/mL of the samples could increase NO production and phagocytic activity of macrophages.