| Objective: The exact function of the lymphatic stomata, is still not clear, but through access to relevant literature, peritoneal lymphatic stomata is indeed involved in substance transportation intra-abdominal process, but whether to participate in lymphatic transport and is in the SIRS, the MODS process plays an important role has not been clearly reported. This experiment mainly investigate the abdominal infection changes the peritoneal lymphatic stomata, and abdominal infection in the body changes in endotoxin concentration in early two ways, aims at a discussion on peritoneal lymphatic stomata as lymphatic drainage pathway, are also involved in the transport of substances of intra-abdominal process, the infection caused by serious systemic response syndrome, MODS also play an important role; the corresponding research and regulation making machine for lymphatic stomata, provide theoretical basis for future use of lymphatic stomata in treatment and prevention of certain diseases. Methods: 60 WISTA rats(200-250g) were randomly divided into normal group(n=10), sham operation group(n=10), abdominal cavity infection group(group CLP)(n=20) and drug intervention group(n=20). The following indexes were measured in rats: 1).the application of SEM observation of the rats peritoneal lymphatic stomata aperture size and distribution density; 2).using the chromogenic substrate Limulus amebocyte lysate test in portal vein blood and thoracic duct lymph endotoxin concentration;3), Nitric oxide nitric acid reduction method determination of the NO content in peritoneal tissues.4).Western blot to detect the expression of nitric oxide synthase(NOS).5). Flow cytometry instrument detection of intracellular [Ca2+] concentration.Results:Compared with the normal control group, intraperitoneal infection lymph pore diameter(P<0.01) and density increased significantly(P<0.01), and the infection time is longer, the peritoneal lymphatic aperture increased more significantly(P<0.01), but the density has not increased significantly(P>0.05), the first drug- intervention, intervention group 2H lymphatic stomata aperture and the distribution density is abdominal infection in 2H group decreased significantly(P<0.01, P<0.01), intervention group 4H lymph hole diameter and distribution density is abdominal infection group 4H still decreased significantly(P<0.01, P<0.05); the abdominal infection in rats of thoracic duct lymph endotoxin content was significantly higher than that in the normal group(P<0.01, P<0.01), and the infection time is more long, the endotoxin content in the thoracic duct lymph is higher(P<0.01), but no significant difference between the content of bacterial endotoxin in rats in the portal vein blood(P>0.05), the first drug- discovery of thoracic duct intervention, endotoxin content was infection group corresponding phase decreases(P<0.01, P<0.01). The content of the infected group rats peritoneal tissue in the abdominal cavity of NO- was significantly higher than the normal group(P<0.01, P<0.01); drug intervention group NO concentration than infection group was significantly lower(P<0.01, P<0.01)), and the treatment group was higher than that of normal group, the concentration of NO(P<0.01, P<0.01). The infection group, the expression level of NOS was significantly higher than that of the normal group(P<0.01, P<0.01) in advance, and after treatment, the expression level of NOS and the infection group at the same time tends to be lower than(P<0.05, P<0.05), but still higher than normal group(P<0.05, P<0.05). The normal circumstances, peritoneal mesothelial cell calcium ions in [Ca2+] at a higher level, infection of abdominal cavity, the calcium ion concentration of [Ca2+] cells was significantly lower than that in normal conditions of intracellular [Ca2+](P<0.05, P<0.05), and drug treatment prior to treatment, the intracellular [Ca2+] concentration was significantly higher than the infection group(P<0.05, P<0.05). Conclusion:1. Abdominal cavity infection affect the morphology change of peritoneal lymphatic stomata, leading to peritoneal lymphatic stomata aperture expanding, the corresponding increase in density distribution.2. Abdominal infection, Bacterial endotoxin by larger, the increasing number of peritoneal lymphatic hole into the lymph circumfluence system may be the main way to cause systemic spread. 3.Peritoneal tissues of NO is an important regulator for regulating lymphatic stomata.4. The NO-c GMP-[Ca2+] pathway may be the main pathway regulating lymphatic stomata. 5.Angiotensin II type 2 receptor specific blocker PD123319 can be the peritoneal lymphatic stomata aperture smaller, reducing the number of open. 6.Perhaps the main cause of leading to higher peritoneal organization NO concentration are associated with increased the expression of e NOS mesothelium cell. |
PDF Full Text Request