Extraction And Activity Of Volatile Oil Of Lindera Setchuenensis And Lindera Nacusua Leaf

Objective In order to explore the extraction procedure and activities of leaves of Lindera setchuenensis (LS) and Lindera nacusua (LN), as well as the interactions between volatile components with biological activities, to expound on the basic prin-ciple and mechanism of the drug property.Methods Find the optimal conditions for volatile oil extraction via single factor and multi-factor experiments and measure the components of volatile oil and its relative amount with GC-MS. Compare the antioxidant functions among the Vc, BHA and extractions, taking the clearance rate of DPPH radicals and the impact on fat and oil POV value as an indicator. The inhibitory activity of extracted volatile oil is veri-fied with filter paper diffusion method.Results 1、Soild-to-liquid ratio of extraction condition of LS is 1:16; LN 1:14. Soak 22h, Ultrasonic impact 30min, keep Slightly boiling state for 5-6h, taking the oil, dissolving in anhydrous ether, natural volatile dry light and get yellow oily liquid, LS extraction rate 0.012%. LS about 0.029%.2、Establishes the GC-MS conditions of chemical constituents of volatile oil; and separated of 79 components in the volatile oil from LS,49 compounds were identified of the total content is 62.03%. relatively high content of components of palmitic acid (25.61%), nutmeg aldehyde (9.81%), phytol (6.61%), linoleic acid (5.39%) and Z-13-eighteen olefine aldehyde (5.38%); separated from the LN oil of the 40 compo-nent, identified 22 compounds, relatively high content of components of caryo-phyllene oxide (8.79%), six hydrogen farnesene acetyl acetone (6.83%), beta selinene (5.02%), palmitic acid (4.42%), neophytadiene (4.53%) etc. And the two species leaves essential oil have only 5 kinds Common contains (alpha pinene, myristic aldehyde, methyl palmitate, palmitic acid, phytol), but the content i s difference.3、The amount of general flavonein the ether extracts from these two species were close, with LS 1.66% and LN 1.43%. And the ether extracts both exert clearance effects on DPPH radicals. The effect of LN with clearance being 66.184% while the LS being 37.842%, which equal to the BHA 4mg·L-1 and Vc 5mg·L4、Under the identical circumstances, the POV value of the blank group of veg-etable oil increases higher than the group of animal oil. The samples is disposed for 48h, POV values increase of vegetable oil is decreasing, the samples activity order is LS power>LN power> Vc> BHA>LS ether extract>LN ether extract. On animal oil POV value was enhanced by increased amplitude order is LN ether extract>LS ether extract> LN powder>LS powder; positive control had only obvious inhibition effect on the POV value of animal oil.5、All the samples to Pseudomonas aeruginosa is insensitive. Against Candida albicans effects:LS essential oil and L. aggregata root ethyl acetate extract are very sensitive (bacteriostatic ring(BR)>2.0mm), LS essential oil, LS ether extract and LN ether extract show the sensitivity (BR= 1.0mm). Against Escherichia coli:LN ether extract, LN petroleum ether, LN ethyl acetate parts show very sensitive (2.0mm< BR <4.0mm), LS ether extract, LN volatile oil and the L. aggregata root petroleum ether fraction are sensitive (BR≈1.0mm), LS essential oil is low sensitivity (BR≈0.5mm). To Staphylococcus aureus:LN volatile oil, LN ether extract, LN petroleum ether and LS volatile oil, L. aggregata root ethyl acetate extract of inhibition is significantly (2.0mm<BR< 2.5mm), LS the ether extract,LN petroleum ether fraction and L. ag-gregata root ethyl acetate fraction sensitive (1.0mm<BR< 1.5mm); other effects are not obvious.Conclusion The proportions of volatile oil in these two species are scarce, thus steam distillation is not appropriate to industrial production. Despite belonging to the same genus, the components in their volatile oil vary significantly. Studies show that the ether extract from these two species exert biological functions involving in elimination of radicals activities and POV value, yet the detailed mechanism is unrav-eled. Further researches are required so as to unveil the antibiosis functions of the ex-tracts.