Studies On The Chemical Constituents Of Lindera Setchuenensis Gamble And Bioactivities Of Compounds From Three Plants Of Lindera

This dissertation is main consisted of two parts. In the first part, the chemical constituents of Linder a setchuenensis Gamble were researched. The ethanol extract of L. setchuenensis was obtained by diacolation method. The ethanol extract was divided into three parts through extracted by petroleum ether, ethyl acetate and n-butanol. The ethyl acetate part was chosen to isolate by various of chromatographic methods, such as silica gel, RP-C18 column, Sephadex LH-20, recrystallization, HPLC semi-preparative method, and so on. And finally,16 compounds were isolated from the ethyl acetate part of L. setchuenensis. Using modern analysis techniques (such as 1H NMR,13C NMR, HR-ESI-MS, HPLC), and combining with TLC method, the structures of 11 compounds had been identified, and they are:β-Sitosterol (1), β-Daucosterol (2), Stigmasterol (3), (-)-Dicentrine (4), Dehydronantenine (5), Demethylmacrosporine I (6),8-hydroxy-9-methoxy-1,2-methylenedioxyaporphine (7), (S)-2,3-dihydroxypropyl undecanoate (8), N-Methylhernangerine (9), Nantenine (10), Phanostenine (11). Compounds 5,7,10 were isolated for the first time from the Lauraceae family, and all the compounds were isolated from L. setchuenensist for the first time.In the second part, some of the alkaloids isolated from L. setchuenensis were chosen to get preliminary anti-tumor activity test. And some compound were found having good anti-tumor activity, it was worthy to get systematic anti-tumor test. With the indication of the preliminary anti-tumor activity test,26 compounds which isolated from three Lindera plants were tested anti-tumor activity by MTT method on five kinds of cancer cells. The results showed that compound 4, JRY09 and JRY10 had good inhibition of cancer cells. Compound 4 inhibited SMMC-7721 and MCF-7 cells with IC50 values of 19.66 μM and 14.99 μM. As to A-549 cells, the inhibition activity was up to 8.09 μM. To HL-60 and MCF-7 cells, the inhibition of Compound JRY09 were 20.9 μM and 17.72 μM. Compound JRY10 showed growth inhibition on SMMC-7721, A-549 and MCF-7 cells, the IC50 values were 17.40 μM,19.16 μM and 19.51 μM, respectively. At the same time, we tested the inhibition activity on fatty acid synthase of compounds WS01, WS02, WS03, WS04, WS05, JRY08, JRY10 in MDA-MB-231 cancer cells. The result showed that JRYIO had strong inhibition on cancer cells and fatty acid synthase with IC5029.17 μM and 43.65 μM. And it showed that JRY10 induced MDA-MB-231 cancer cells apoptosis by inhibiting FAS activity.