Experimental Study Of Neural Stem Cell Transplantation In The Treatment Of Cerebral Infarction In Rats

Background At present, cerebral infarction(cerebral infarction) is the leading cause of human death and disability of the main diseases that endanger human health of. At present, cell damage and death caused by cerebral infarction is still no effective treatment. Once the ischemic injury of cerebral infarction can reach the maximum, can lead to irreversible neurological damage, to recover before the onset of the condition is very difficult. Even get timely treatment, many patients still lack permanent neurological function, left with varying degrees of disability. In recent years, researchers at home and abroad found a variety of treatment options, minimally invasive treatment from thrombolytic therapy, from the application of a variety of drugs or Chinese traditional medicine, to restore cerebral blood supply, protect the ischemic penumbra of brain tissue. Although these methods can improve the clinical symptoms of the patients, but because of the restriction of the "time window" and many other factors, can not fundamentally block brain injury or effectively promote damaged brain tissue regeneration. Therefore, the repair of damaged brain tissue and nerve function recovery is an ideal treatment for the people to pursue. Experimental study found that cell transplantation, through its potential self repair damaged tissue is born. In view of this, the researchers focus on how to use stem cell transplantation in the treatment of cerebral infarction. Stem cell transplantation for treatment of cerebral infarction, is through the promotion of local variety of neurotrophic factors; these factors can be reduced by scavenging free radicals, play the toxicity of excitatory amino acid in various ways neuroprotective effect, so as to reduce the occurrence of apoptosis.Through the promotion of vascular endothelial growth factor(VEGF), basic fibroblast growth factor(b FGF) and other cytokines and stimulate angiogenesis, enhance regional cerebral blood flow and ischemia injury. Therefore, this experiment selects a complete individual differentiation potential of embryonic stem cells transplanted into the rat model of cerebral infarction, and explore the curative effect and in vivo proliferation, differentiation.Objective To investigate the feasibility and effect of neural stem cell transplantation for the treatment of neurological injury in rats with cerebral infarction.Methods The adult made the model of middle cerebral artery in male rats with the suture method, rats were divided into experimental group 25 and control group 25, 15 d from pregnant fetal rat cerebral cortex and hippocampus was extracted from cultured nerve cells, the positive expression of nestin was identified as neural stem cells, the experimental group by intravenous injection of 1 μL neural stem cell suspension, the control group by intravenous injection of 1 μL normal saline, nerve function in rats of the 18 score in 1d after transplantation, 3d, 7d, 14 d, 28 d according to the Garcia score, cerebral perfusion and fixation materials, through cell differentiation, migration and integration of neural stem transplantation after immunohistochemistry method.Results The cultured nerve cells in serum-free medium to form a large number of neurospheres 1 in vitro. After 3- 5 generations passaged cells grew stably. The nestin staining is positive in most cells, neurospheres by fetal bovine serum culture medium after adherent culture can be divided into the expression of neuron specific enolase(NSE), glial fibrillary acidic protein(GFAP) and galactocerebroside(GALC) positive cells.2.The scores of neurological function in rats after transplantation: nerve ischemia transplantation group rats were significantly increased compared with the control group(P<0.05); nerve function ischemia transplantation group rats were significantly increased in 28 d compared to 14d(P<0.05). The results showed that ischemia transplantation group compared with the control group, the neurological function score was significantly increased, the differences were significantly different(P<0.05).3.Immunofluorescence staining, immunohistochemical staining to detect Brdu positive cell survival after transplantation, 28 d grafted cells migrated to the surrounding, differentiation, angiogenesis, and vascular hyperplasia visible edge of the infarct area. The experimental group rats were GFAP positive cell count increased than the control group, the difference was statistically significant(P<0.05).Conclusion 1.Isolated from day 15 of gestation fetal rat cerebral cortex and hippocampus neural stem cells have self-renewal and multilineage differentiation potential, in the liquid of neural stem cells into neurons and glial cells have potential to differentiate the cultivation of 5% fetal bovine serum.2.Neural stem cell transplantation, migration, to around 28 d visible differentiation of transplanted cells, participate in the formation of blood vessels, and the visible edge of infarcted region vascular hyperplasia.3.In vitro culture of neural stem cells can survive, migrate and differentiate within cerebral infarction in rat brain.4.In vitro culture of neural stem cells transplanted into rats after cerebral infarction, and the rehabilitation of neurological function in cerebral infarction rats has played a positive role.