Expression And Clinical Significance Of HIF-1α And VEGF In The Non-small Cell Lung Cancer

ABSTRACTObjective: The aim of the study is to detect the expression and correlation of HIF-1α and VEGF, and to evaluate the clinical significance and correlation of expression of HIF-1α and VEGF in NSCLC. To analyze the correlation of the expression of HIF-1α and prognosis of patients.investigate the correlation and clinical significance between and angiogenesis in non-small cell lung cancer(NSCLC) of human, observe the relationship of HIF-1α, VEGF with clinicopathological parameters, observe the expression of HIF-1α, the significance and prognostic impact analysis in NSCLC tissues, to preliminarily analyze the relationships between the expression of HIF-1α and tumor cell apoptosis in NSCLC.Methods: using immunohistochemical SP method, to observe the expression of HIF-1α and vascular endothelial growth factor(VEGF) in the NSCLC specimens of 95 patients, to observe the relationships of HIF-1α with clinical features related factors By chi-square test and Fisher’s exact test method, Univariate analysis HIF-1α and 3-year survival of patients by Kaplan-Meier method, COX multivariate regression analysis of factors affecting the prognosis of the disease, to observe the apoptosis of tumor cells by using flow cytometry.Results:1 The positive expression rate of HIF-1α and VEGF was 73.9%(68/92) and 63.0%(58/92) in 92 cases of NSCLC tissues, and was in peritumoral tissue the positive expression rate of 39.1%(36/92), 23.9%(22/92). Differences were significant(P<0.05).2 In the group of HIF-1α of low expression, the expression of HIF-1α and VEGF in NSCLC were not correlated with gender, age, smoking history, lymphatic metastasis, TNM stage, grading or histologic subtype. Differences were not significant(P>0.05). In the group of HIF-1α of high expression, the expression of HIF-1α and VEGF in NSCLC were not correlated with gender, age, smoking history or histologic subtype. Differences were not significant(P>0.05). Lymphatic metastasis, TNM stage, grading were closely correlated with its expression. Differences were significant(P<0.05).3 In the patients of NSCLC, the low expression of VEGF was 27 cases, the high expression of VEGF was 31 cases. The results show that the expression of HIF-1α was positively correlated with VEGF, in the group of HIF-1α of high expression, the expression of VEGF increased correspondingly. Differences were significant(P<0.05).4 About 3 years survival rate of patients, the results show that the group of HIF-1α of high expression was 40%, the group of HIF-1α of low expression was 70%. Differences were significant(P < 0.05). Prognosis factors of disease include age, lymphatic metastasis, TNM stage, grading and the expression of VEGF(P<0.05). COX multiple factors regression analysis results suggest that lymphatic metastasis, TNM stage or HIF-1α can influence alonely the prognosis of disease(P<0.05).5 By using flow cytometry to analyze cell apoptosis, the results show that the expression of HIF-1α was negatively correlated with the situation of cell apoptosis. Comparing with the low expression, in the group of high expression, tumor cell apoptosis rate is significantly decreased. Differences were significant(P<0.05).Conclusion:1 In the NSCLC tissue, the expressions of HIF-1α and VEGF were significantly higher than those of the corresponding lung tissue adjacent to carcinoma and the expression of HIF-1α was positively correlated with the expression of VEGF.2 In the NSCLC tissue, the expression of HIF-1α was closely associated with lymphatic metastasis, TNM stage, grading, HIF-1α and above factors can influence the prognosis of disease independently3 The negative correlation between the expression of HIF-1α and situation of cell apoptosis in tumor. That show that decreasing the expression of HIF-1α can can block the growth and metastasis of tumor cells at the molecular level, and provide a new target for the treatment of NSCLC.