| IntroductionThe role of angiogenesis in the growth and metastasis of solid tumor has been clearly established. Angiogenesis in the tumor is usually expressed by in-tratumoral microvessel density (iMDV) , which is an important factor that holds the key to the size and metastasis of solid tumor and the prognosis of patients. Some studies found that angiogensis is associated with vascular growth factor excreted and activated by tunor cell, vascular endothelial growth factor( VEGF)/ vascular permealility factor ( VPF) can alter microvascular permealility, and stimulate endothelial cell migration and proliferation. It is currently considered the most relevant and the most endothelium spcific of the already known endothelial growth factors.Tumor is a species of cell periodic disease, the entity of malignant tumor lies in that the adjestment of cell cycle is out of control , the cell report the unlimited independence proliferation and cleavage. Under the feat incitement factor ( such as the cancer gene, growth factor) function, the cell were activated to enter to the proliferational appearance from the static appearance. The cell pro-liferatim index( PI) mean that the cell the proliferational appearance, its value is more high to hint the tumor cell propagates more quick.Recently, domestic and international studies reported the role of VEGF and angiogensis in the episode, therapy and prognosis of small cell lungcancer ( SCLC) , but to SCLC the research is seldom. In this experiment we detected the expression levels of VEGF in 33 SCLC specimen and its association with angiogensis, PI and clinical data using the immunocytochemistry technique andflow cytometry(FMC) technique, to define whether there is high VEGF expression in the SCLC, and VEGF and angiogensis whether to rise important role in the episoded process of SC1C or not .Material and method1. Clinical casesCase: 33 patients with SCLC who consecutively underwent curative surgical resection at the second hospital affiliated to China medical university from Janna-ry 1995 throught June 2003 were studied They had not received either chemotherapy or radiation therapy before surgery. The pathologic features of the samples were classified according to World Health Organization histologic criteria and to guidelines of the American Joint Committee on Cancer staging. There were 20 male patients and 13 female patients. A detailed medical history included the following: age, sex, tumor size, node status, TNM stage, etc. The tunor samples were fixed in formalin and embedded in paraffin for histologic and im-munohistochemical processing.Group: due to limited cases, I II were assigned as Early and III IV as Late; Tumor size was divided tow groups according to 3cm .Contrast; 8 embedded in paraffin samples of lung benign tumor2. Detecting VEGF expression by immunohistochemistryWe examined the VEGF expression status using SP method. Posituve cells were dyed buffy which specificly located at cell plasm or nucleus.3. Micro vessel density in tumor (MVD)An anti - CD34 monoclonal antibody, which is specific for endothelial cells, and immunohistochemical techniques were used to measure angiogensis in tumor samples. Weidener'method was used to count microvessel.4. Cell proliferation period analysis by flow cytometrySCLC cells fixed in 70% alcohol were dyed with PI following RNAase treat-ment. Cell proliferation periods were analyzed by FCM, and the results were ex-pressed as proliferation index (PI).5. Statistical methods: statistical analysis were performed using statisticalSPSS 11.5 software, x2 test,analysis of variance,rank sum test,Spearman correlate were used.Results1. VEGF expression detected by immunocytochemistry.VEGF positive rate was significantly higher in SCLC group than in control group [63. (34% (21/33) vs. 12.50(1/8)], P<0.05.2. iMVD measured by anti - CD34 monoclonal antibodyiMVD measured by anti - CD34 monoclonal antibody in SCLC group was significantly higher[ 18.70 149. 30(M = 67.23) /200...
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