Protective Effects Of QBFFTSG On The Experimental Burns In Rats

The Burn is one of the most common traumatic diseases caused by physical or chemical factors, such as thermal, chemical, electric current, and radiation. Large-area burns can disrupt the function, metabolism and morphology of our body and result in serious problems, even damage the internal organs. Burn can also increase the incidence of shock, sepsis and multiple organ function failure, correlarating with relatively high mortality rate in recent years. Compound burn ointment(Qin bai fu fang tang shang gao,QBFFTSG), a folk secret recipe of Chinese traditional medicine, composed of radix astragali, radix scutellariae and giant knotweed, is used for clinical treatment of burn.According to the clinical experience, the QBFFTSG can promote saprophytic muscle regeneration, prevent inflammation, bacterial infection, and contribute to the regeneration of skin cells. In our study, we mainly focus on exploring the protective role of the QBFFTSG and the potential mechanism in the rat experimental burn model,making a greater contribution to the promotion of the wide application of compound burn ointment.Aim The protection effects of the QBFFTSG and the potential mechanism on the rat experimental burn model.Methods1. Subcutaneous injection of carrageen glue to build the rat paw swelling model. SD rats were randomly divided into model group, the Matrix group(vaseline), positive control group extract crude drugs(moist burn cream 0.32 g crude drug/g extract), FFTSG(0.4,0.2,0.1g crude drug/g extract), each group received the appropriate dose of drug.After seven days, The changes of rat foot swelling degree were evaluated by injecting the carageen glue and measure the foot plantar volume.2. Wipe xylene inflammatory reagent to build the mouse ear swelling model. Kunming mice were randomly divided into 6 groups: model group, the Matrix group(vaseline),positive control group(moist burn cream 0.64 g crude drugs /g extract), the QBFFTSG groups(0.8, 0.4, 0.2 g crude drugs/g extract), wipe the dimethylbenzene on the surface of mice ears, and treat the mouse with drugs indicated above, weighing the mice to measure the thickness of each ear and calculating the swelling rate.3. Using the hot plate method to build mice scald model. Kunming mice were randomly divided into six groups: model group, the matrix group(vaseline), positive control group(moist burn cream 0.64 g crude drugs/g extract) and QBFFTSG groups(0.8, 0.4,0.2 g crude drugs/g extract), model group(Natural Saline), the rest of the group daub foot plantar with corresponding drugs, hot plate method was used to measure the pain threshold of mice at different time points.4. Use warm water bath method built the â…¡ degree of mice scald model. Sixty SD rats were randomly divided into six groups: model group, the matrix group(vaseline),positive control group(moist burn cream 0.32 g crude drugs/g extract), the QBFFTSG(0.4, 0.2, 0.1 g crude drugs/g extract), remove the back hair and anesthesia after disinfection, warm bath the mice back in 80 degree constant temperature bath pot, inject the corresponding drug for 20 days after burning continuously. Measure the scabby area and the scab healing time in the fifth, tenth and fifteenth day.5. Use hydrochloride acid method to build rats experimental burning model. Thirty male SD rats and thirty female, weighed 180-220 g, were divided into six groups: model group, the group matrix(vaseline), positive control group(moist burn cream 0.32 g crude drugs/g extract), the QBFFTSG group(0.4, 0.2, 0.1 g crude drugs/g extract), wipe hydrochloricacid, inject the drugs indicated above for 20 days every day after burning,Measure the scabby area and the scab healing time at different time points.6. Use alcohol burning method to build burning model. 70 SD rats, randomly divided into 7 groups: untreated group, model group, the matrix group(vaseline), positive control group(moist burn cream 0.32 g crude drugs/g extract), the QBFFTSG group(0.4, 0.2, 0.1 g crude drugs/g extract), remove the back hair and anesthesia after disinfection, burn mice with alcohol, after that inject the drugs indicated above for 20 days. Using dry-wet method to measure the wound moisture, graphical weighing method to measure the healing degree of wound and use H&E staining to observe the pathological changes of the wound.Results: The high, medium and low dose of QBFFTSG can significantly reduce the paw and ear swelling degree in the rat experimental model. Both high and middle dose of FFTSG can obviously increase the threshold of pain in mice. In the experimental scalded rats, both high and middle dose of QBFFTSG can significantly reduce the wound scabby area ten days and fifteen days after the drug was administrated and shorten the scab healing time; Moreover, the high, medium and low dose of QBFFTSG could significantly increased the degree of wound healing, the high and the middle dose group showed a reduced water content of skin in the trauma and improved the pathological changes of the wound.Conclusion: QBFFTSG can reduce skin edema caused by the burns, promote wound healing and may function as anti-inflammatory and analgesic drugs.