| Objective: With the increasingly progress of our aging society, the incidence of coronary heart disease has gradually increased. Therefore, the study of the etiology and mechanisms of coronary heart disease has become a critical issue. Both platelets and macrophages play an important role in the pathogenesis of atherosclerosis. The microparticles derived from platelets carrying a variety of components may contribute to atherosclerosis. The aim of this study is to investigate a possible release of microparticles from activated platelets and to clarify the interaction of platelet microparticles and macrophage throughout atherosclerosis process.Method: We separate microparticles by ultracentrifugation of activated platelets, then investigate its biological function using flow cytometry and other detection methods. Intake of Ox-LDL is tested to clarify the influence of platelet microparticles to phagocytic capacity. And Inflammatory cytokines secretion of macrophage are measured by ELISA.Results: The microparticles are isolated and identified successfully from activated platelet. Macrophage can phagocytose platelet microparticles, which increase the intake of Ox-LDL significantly(P<0.05). PMP enhanced secretion of inflammatory cytokines IL-1β and TNFα in the first 2-4h(P <0.05), and secretion of inflammatory cytokines IL-6 enhanced in the first 2h(P <0.05).Conclusion: When microparticles released from activated platelets enter macrophages, biological activity and release of inflammatory factors can be enhanced. So we conclude that PMPs are pro-inflammatory factors, which may contain abundant bioactive components such as mi RNAs, m RNAs, DNAs and proteins. |
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