| Background: Lung cancer is the main cause of cancer death in both men and women all over the world, accounting for 25% of all cancer deaths. The lung cancer is a serious threat to human life, being the first of cancer in morbidity and mortality in the world. Omega-3 PUFAs is a kind of human body essential fatty acids in human body, and is closely related to our health. Omega-3 PUFAs has some adjustments in human cardiovascular system, immune system and nervous system. In recent years, the antitumor function of the omega-3 PUFAs has always been a research focus.There are more and more evidences that omega-3 PUFAs plays an important role in the risk of cancer and development. The anti-tumor research about omega-3 PUFAs is mainly focused on gastric cancer, breast cancer and prostate cancer, but only a few researches about the omega-3 PUFAs`s effects on the anti-lung cancer fields.Objective:So far, we don`t know whether omega-3 PUFAs can induce apoptosis on lung adenocarcinoma cancer cells line H1975 and H460, and whether the mechanisms of apoptosis are associated with the changes on survivin expression in lung adenocarcinoma cancer cell. This study mainly aims at discussing the problems above.Methods: In this study, the untreated group was defined as a negative controlgroup, and the cells with gemcitabine was defined as the positive control group. Omega-3 PUFAs(EPA and DHA) treated were defined as treatment groups. In treatment groups, Omega-3 PUFAs(EPA and DHA) was used to process lung adenocarcinoma cancer cells line H460 and H1975. Different doses(25, 50, 100, 150, 200, 300 μmol/L) of EPA and DHA were used to deal with human lung adenocarcinoma cancer cells line H460 and H1975 for 24, 48, 72 hours. After the process, CCK- 8 was used to test their activity.Flow cytometry was used to detect the effect of omega-3PUFAs on those cell apoptosis rate after being dealed on different doses(100, 200, 300 μmol/L) of EPA and DHA. The effect of omega-3PUFAs on cell cycle was detected by flow cytometry after 200 μmol/L of EPA and DHA(100, 200 μmol/L) being used to deal with those cells for 24 hours. After different doses(100, 200, 300, 200 μmol/L) of EPA and DHA to deal with them for 24 hours, western blot was used to detect the expression of survivin protein and verify the effectiveness after over-expressing survivin. CCK-8 was used to detect the cell activity after overexpressing survivin.Results: Omega-3 PUFAs can inhibit the cell activity, and a negative correlation was observed on the activity and omega-3 PUFAs doses. The apoptosis rate had a significant difference between the treatment group and the untreated group, with statistical significance( P < 0.05). Low concentration omega-3PUFAs had no obvious effect on those cells, but high concentration omega-3PUFAs can induce cells apoptosis with statisticalsignificance(P<0.05). The cell cycle rate had no obvious effect during those groups. We discovered that survivin can express in human lung adenocarcinoma cancer cell lines H460 and H1975 by western blot method. Survivin was highly expressed in negative control group compared with negative control group,and the expression of survivin had more obviously decreased after giving a high dose of omega-3 PUFAs. The expression of Survivin in H1975 and H460 cells obviously decreased when treated with omega-3 PUFAs. After increasing expression of survivin, the tumor cell activity significantly increased compared with negative control group(p<0.05).Conclusion: Omega-3 PUFAs(EPA and DHA) can inhibit cell activity and promote apoptosis of Lung adenocarcinoma cancer cells line H1975 and H460 cells,and the mechanism maybe associated with the changed of Survivin expression after treated by omega-3 PUFAs in human lung adenocarcinoma cancer cells line H1975 and H460. |
