ApoM Inhibites The Expressions Of ICAM-1 And VCAM-1 MRNA Induced By LPS In Mice

Objective To evaluated the model of LPS-induced inflammation in apolipoprotein M(Apo M) gene knockout(Apo M-KO) mice and to investigate the effect of Apo M on the expression of ICAM-1 and VCAM-1 m RNA in mice liver.Methods: Twelve wild-type(WT) or Apo M-KO mice which were identified by the base-quenched probe technique were randomly divided into control(Normal saline, NS) group and treatment(LPS) group. The mice were intragastrically administered with NS(0.1ml) or LPS(10mg/kg dissolved in NS) for 3 days. Mice were sacrificed 3 days later and real-time PCR was adopted to detect the expressions of ICAM-1 and VCAM-1 in mice liver.Results: Administration of LPS to WT and Apo M-KO mice led to an increased expression of ICAM-1 in the liver, but the magnitude of this induction was greater in Apo M-KO mice compared to WT mice. Compared with the control group, the treatment group of Apo M-KO mice showed significantly higher expression of VCAM-1 but not WT mice. Compared to the WT mice, Apo M-KO mice showed inceased level of VCAM-1 after treatment with LPS. TWO-Way ANOVA showed that there was interaction between LPS and Apo M on ICAM-1 and VCAM-1 expression levels in mice liver.Conclusions: Our data suggest that Apo M inhibited LPS-induced ICAM-1 and VCAM-1 expression in mice liver.