Effect Of Angiotensin(1-7) On The Expression Of ICAM-1,VCAM-1 And LOX-1 In The Endothelial Cells Of Rat Aorta Induced By Ox-LDL

Objective:Atherosclerosis is the most common diseases of cardiovascular system diseases, also is one of human health hazard of common diseases, mainly in vessel wall endothelial cells injury leading to lipid accumulation, affect inflammatory cell aggregation was characterized. The oxidized low density lipoprotein is one of the important risk factors,oxLDL in endothelial cell dysfunction or damage to its specific receptors on the surface of hemagglutinin sample oxidized low density lipoprotein receptor,Inducing the formation of foam cells and start the AS plaque formation and development.In the process, vascular cell adhesion molecule-1, intercellular adhesion molecule-1 are two important adhesion molecule, participate in a variety of pathological and physiological process, which can cause endothelial dysfunction of anticoagulant and anti adhesion, accelerate the AS form.Ang-(1-7) is a new member of the renin-Angiotensin system.Ang-(1-7) has the expansion blood vessel and antiproliferative.which have various protection and the adjustment function on the cardiovascular system.This study intends to in vitro culture of rat aortic endothelial cells(SVAREC), ox-LDL induced by different concentration and on the basis of ox-LDL to join Ang-(1-7) induced, were observed before and after endothelial cell proliferation and LOX-1, ICAM-1, VCAM-1 transcription level of dynamic change and Ang-(1-7) possible mechanism to protect the endothelial cells.For a more in-depth understanding atherosclerosis the molecular mechanism of formation and find drug with potential molecular targets.Methods:1.The experimental Cell Counting Kit(CCK 8) detection by the ox- LDL group and ox- LDL + Ang-(1-7) group SVAREC Cell proliferation after processing.2.The experiment with fluorescent quantitative polymerase reaction(RT-PCR) method to detect the ox-LDL group and ox-LDL + Ang-(1-7) treatment after SVAREC cells of LOX-1, ICAM-1, VCAM-1 m RNA expression level.Results:1.Using Cell Counting Kit(CCK-8) test results show that: ①ox- LDL groups: adding different concentrations of ox-LDL( join10 mg/L, 20 mg/L, 50 mg/L, 100 mg/L) and SVAREC Cell group training after 24 h, has obvious inhibitory effect on SVAREC Cell proliferation.In the concentration range of 0-100 mg/L, ox-LDL group cell proliferation inhibition rate increased with the increase of drug concentration ox-LDL and in dose-dependent, compared with control group, with statistical significance(P <0.05).②The ox-LDL + Ang-(1-7) groups: experimental group joined the ox-LDL concentration of 100 mg/L, respectively to join again Ang-(1-7) concentration for 10-9mol/L, 10-8mol/L, 10-7mol/L, 10-6mol/L and SVAREC cell group training after 24 h,with the rise of Ang-(1-7) concentration, SVAREC cell growth inhibition rate is reduced,dose dependent, compared with control group, the difference was statistically significant(P< 0.05).2.Experiment with fluorescent quantitative polymerase reaction(RT-PCR) method to detect the results showed:(1) ox-LDL groups: adding different concentrations of ox- LDL(10 mg/L join, 20 mg/L, 50 mg/L, 100 mg/L) and SVAREC cell group training after 24 h.In the concentration range of 0-100 mg/L, with the increase of drug concentration of ox-LDL,LOX-1, ICAM-1, VCAM-1 m RNA expression levels increase, compared with control group, with statistical significance(P < 0.05).(2) The ox- LDL + Ang-(1-7) groups:experimental group joined the ox-LDL concentration of 100 mg/L, respectively to join again Ang-(1-7) concentration for 10-9mol/L, 10-8mol/L, 10-7mol/L, 10-6mol/L andSVAREC cell group training after 24 h, with the rise of Ang-(1-7) concentration, with the increased concentration of Ang-(1-7), LOX-1, ICAM-1, VCAM-1 m RNA expression level decreased obviously, compared with control group, with statistical significance(P < 0.05).Conclusion:1.The ox- LDL can inhibit the growth of SVAREC cells, Ang-(1-7) to reverse the ox-LDL caused by cell growth inhibition.2.ox- LDL can make LOX-1, ICAM 1, VCAM-1 m RNA expression levels increase and in dose-dependent, Ang-(1-7) make LOX-1, ICAM 1, VCAM-1 m RNA expression levels decrease and in dose-dependent,. Prompt ox- LDL in endothelial cell dysfunction or damage to its specific receptors on the surface of LOX-1, promote ICAM-1 and VCAM-1the expression of a series of inflammation, immune response, thus promotes the formation of the AS, and Ang-(1-7) can reverse endothelial injury caused by ox- LDL, Ang-(1-7)has a protective effect on endothelial cells.