Effect Of Methimazole On ERS Induced ICAM-1?VCAM-1 Expression In Thyroid Cells

ObjectiveTo investigate the effects of anti-thyroid drug Methimazole on the expressions of ICAM-1 and VCAM-1 in human normal thyroid cells induced by endoplasmic reticulum stress,and to study the effects of Methimazole on endoplasmic reticulum stress proteins.MethodsUsing T25 cell culture flask cultivate normal thyroid cell line?Nthy-ori-3-1?in37?5%CO₂ incubator untill logarithmic growth phase,then transfer cells to six-well plate to cultivate.The cell will be experimental object when they touch each other and fusion degree up to 100%.Thyroid cells were treated with endoplasmic reticulum stress agonist Tunicamycin?TM?and anti-thyroid drug Methimazole?MMI?,and the cell were divided into four groups:Control group 1,Control group 2,Tunicamycin group,Tunicamycin+Methimazole group.Proteins were extracted after intervention,then detected the expression of proteins of ICAM-1,VCAM-1,PERK,IRE1,ATF6 and GRP78 by western blot.Results1.The relative expression levels of ICAM-1 in tunicamycin group,control group 1and control group 2 were 0.457�0.035?0.077�0.015 and 0.113�0.024;The relative expression levels of VCAM-1 in tunicamycin group,control group 1 and control group2 were 0.490�0.116?0.053�0.024 and 0.093�0.034;The relative expression levels of PERK in tunicamycin group and control group 1 and control group 2 were 0.825�0.075?0.090�0.021 and 0.137�0.037;The relative expression levels of IRE1 in tunicamycin group and control group 1 and control group 2 were 0.630�0.085?0.083�0.023 and0.141�0.0361;The relative expression levels of GRP78 in tunicamycin group,control group 1 and control group 2 were 1.700�0.150?0.176�0.540 and 0.222�0.122;Therefore,?1?the expression levels of ICAM-1 and VCAM-1 in tunicamycin group were significantly higher than those in control group 1 and control group 2?p<0.05?,and the expression levels of endoplasmic reticulum stress related proteins?PERK,ATF6,IRE1,GRP78?were also significantly increased?p<0.05?.?2?there was no significant difference in expression of ICAM-1,VCAM-1,PERK,IRE1,ATF6,GRP78 between control group 1 and control group 2?p>0.05?.2.The relative expression levels of ICAM-1 in tunicamycin+methimazole group and tunicamycin group were 0.180�0.017 and 0.387�0.041;The relative expression levels of VCAM-1 in tunicamycin+methimazole group and tunicamycin group were0.160�0.036 and 0.413�0.041;The relative expression levels of PERK in tunicamycin+methimazole group and tunicamycin group were 0.120�0.050 and 0.963�0.073;The relative expression levels of IRE1 in tunicamycin+methimazole group and tunicamycin group were 0.100�0.040 and 0.550�0.029;The relative expression levels of ATF6 in tunicamycin+methimazole group and tunicamycin group were 0.430�0.053 and0.873�0.136;The relative expression levels of GRP78 in tunicamycin+methimazole and tunicamycin group were 0.277�0.058 and 1.773�0.114;Therefore,?1?the expressions of ICAM-1,VCAM-1 in the tunicamycin+methimazole group were significantly lower than those in the tunicamycin group?p<0.05?,and the expressions of endoplasmic reticulum stress related proteins?PERK,ATF6,IRE1,GRP78?in the tunicamycin+methimazole group were also significantly lower than those in the tunicamycin group?p<0.05?.?2?There was no significant difference in expression of ICAM-1,VCAM-1,PERK,ATF6,IRE1,GRP78 between control group 1 and control group 2?p>0.05?.Conclusions1.Endoplasmic reticulum stress induced by Tunicamycin promotes the expression of ICAM-1 and VCAM-1 protein in thyroid cells.2.Methimazole inhibited the expression of ICAM-1 and VCAM-1 protein induced by endoplasmic reticulum stress,as well as the expression of endoplasmic reticulum stress related proteins PERK,IRE1,ATF6 and GRP78.Methimazole can inhibited the expression of ICAM-1 and VCAM-1 proteins by inhibiting the pathway of endoplasmic reticulum stress.