Adjusting Heart And Kidney Prescription Inpact On Mice With Mild Cognitive Impairment Learning And Memory Ability And Brain Tissue The Content Of MDA、the Activity Of SOD

ABSTRACTObjective: To observe adjusting heart and kidney prescription inpact on Mice with mild cognitive impairment learning and memory ability and Brain tissue The content of MDA、The activity of SOD,To explore the adjusting heart and kidney prescription Mechanism of therapeutic efficacy of mild cognitive impairment.Methods: Small mice fast food after 16 hours respectively into the maze of food,choose mice always looking for the run, and 20 to 40 minutes to find food for experiment mice.To return 15 mice do normal control group,The other mice subcutaneous injection 10% D-galactose 100mg/kg once a day,for eight weeks。After the success of the building,the model mice were randomly divided into 5 groups,Including the model control group, positive drug group(compound brain protein hydrolysate)300mg/kg, and adjusting heart and kidney prescription high dose group(13.0g raw medicine/kg),middle dose group(6.5g raw medicine/kg),low dose group(3.25 g raw medicine/kg)。Normal control group and model the control group to fill the stomach of distilled water 10 ml/kg daily,the other group lavage give corresponding treatment one times a day for 60 days。After the last delivery mice will once again into the maze of food, to observe and record for five days mice in the maze of food walking direction error times and the time to find food。Then put to death in mice,immediately take whole brain,with The method of Bradford color determination of brain tissue protein content, with colorimetric method determination of MDA content and SOD activity in brain tissue in mice.Results:①Positive drug control group and adjusting heart and kidney prescription high、middle、low dose group all can improve the learning and memory ability of model mice,among them adjusting heart and kidney prescription high dose group decrease in mice to find food error times better than positive drug control group,adjusting heart and kidney prescription middle group In the aspect of shortening the time of mice used in search of food is better than that of positive drug group。②Positive drug control group and adjusting heart and kidney prescription high、middle、low dose group all can decrease MDA content and improving SOD activity in the model mice brain tissue,among them adjusting heart and kidney prescription middle group is better than that of positive drug group。Conclusion:Adjusting heart and kidney prescription can improve cognitive impairment caused by D-galactose ability of learning and memory in mice,the mechanism May be related to improve brain tissue antioxidant capacity,reduce oxidative stress injury,mainly displays in the lower MDA content and improving SOD activity in brain tissue。...