Association Study Between Aldehyde Dehydrogenase2Genotype And Coronary Artery Disease

Coronary heart disease as a common and serious threat to the life andhealth of the patient. With the deepening of research on genes, humanunderstanding of coronary heart disease has been extended to the geneticlevel. Aldehyde dehydrogenase2(ALDH2) is one of the ALDH familymembers, and alcohol dehydrogenase (ADH) joint action will eventuallyenter the body metabolize ethanol acetic acid. In addition, ALDH2alsoprotect the heart, participation nitroglycerin (GTN) biotransformationprocesses. ALDH2presence of a single nucleotide polymorphismmutation, when the outer exon12allele G (guanine) is mutated to A(adenine), the protein encoded by the gene will ALDH2converted toGlu504Lys, significantly reducing ALDH2enzymatic activity, and thushave a major effect on the biological function of ALDH2. ALDH2mutation combinations currently divided into wild-type homozygotesGG, GA heterozygous mutation type, homozygote AA type three, threesuccessive lower activity. Has been reported ALDH2genotype andcoronary heart disease, there is a correlation with acute myocardialinfarction. ALDH2gene may be involved in the development ofatherosclerosis and CAD through a variety of mechanisms. This studycase-control study ALDH2genotype distribution, so as to explore therelationship with ALDH2genotypes and coronary atherosclerosis, coronary heart disease, acute myocardial infarction also ALDH2genotypes and coronary lesion severity and number of Coronary arteryrelevance, are reported below.Abstract: Objective: To explore the correlation between aldehydedehydrogenase2genotype and coronary artery disease. Methods: Atotal of177subjects hospitalized in our hospital from May2013to May2014were selected for the research, including36cases of coronaryatherosclerosis patients;70cases of coronary heart disease patients;25cases of acute myocardial infarction (AMI) patients and46cases in thecontrol. The clinical basic data were recorded. The levels of TC, TG,HDL-C and LDL-C were tested by enzymatic determination. The bloodsamples of all the selected subjects were collected for the extraction ofgenomic DNA, and ALDH2gene was detected by direct sequencing.Results: The distribution of ALDH2genotype in AMI group and controlgroup was in Hardy-Weinberg equilibrium. The genotype frequencies ofGG, GA+AA in experimental group were as follows: coronaryatherosclerosis72.22%、28.78%； coronary heart disease71.43%、28.57%；acute myocardial infarction80%、20%，and the control groupwere:65.22%、34.78%. The difference of three genotypes in the twogroups was not statistically significant (P=0.622). Multi-CategoryLogistic regression analysis showed that the risk of diabetes, smoking,and BMI was the risk factor for AMI, the genotype was not. Conclusions: The risk of diabetes, smoking, and BMI was the riskfactor for AMI. Genotype of ALDH2was not the risk factor for coronaryatherosclerosis, coronary heart disease and AMI.ALDH2genepolymorphism had no relationship with the severity and number ofcoronary artery.