Correlation Of Plasma Vitamin D Levels With Prognosis Of Advanced Non-small-cell Lung Cancer

Objectives：To investigate the association between plasma vitamin D levelsand the prognosis of advanced non-small cell lung cancer patients.Methods:1. A retrospective analysis of newly diagnosed advancednon-small cell lung cancer patients in Cancer Center，First Hospital, JilinUniversity，between March1st,2011and July1st,2014. A total of195caseswere enrolled in this study.137cases (70.3%) were male, and58cases (29.3%)were female, the ratio of male to female was2.36to1. The median ages were57years (ranged from34years to75years).2. Three to five milliliters of peripheral venous blood were drawn from eachpatient at the time of initial diagnosis, the blood was centrifuged,100μl plasmasamples were separated in the EP tube, and stored in－80℃freezers, andvitamin D levels were measured by API5500QTRAP LC/MS/MS massspectrometer with LC-20AD HPLC. Then we collect the clinical data of eachpatient, and calculate the progression-free survival of patients (PFS), overallsurvival (OS).3. The clinical distribution of vitamin D levels were analyzed by Chi-squaretest. The prognosis of patients date were analyzed using Kaplan-Meier survivalanalysis, Log-Rank test, Cox proportional hazards regression model.Results：1. Plasma vitamin D levels<20ng/ml148cases(75.9%), plasmavitamin D clevels≥20ng/ml47cases (24.1%).2. The distribution of plasma vitamin D levels was not correlated withgender, age, ECOG scores, BMI index, smoking status, histological type andclinical stage. 3. Vitamin D levels was not correlated with the PFS, we found that age andclinical stage were associated with the PFS, but the gender, smoking status,pathological type and first-line chemotherapy were not correlated with it.4. Vitamin D levels was not correlated with the OS, we found that first-linechemotherapy and multi-line treatment were associated with the OS, but thegender, age, smoking status, pathological type clinical stage were not correlatedwith it.5. Age and clinical stage were independent prognostic risk factors in theadvanced NSCLC patients’ PFS. Pathological type and multi-line treatment wereindependent prognostic risk factors in the advanced NSCLC patients’ OS.6. In the stratified analysis of PFS and OS, on the basis of gender, age,smoking status and histological type, it showed that vitamin D levels was nocorrelated with PFS in all the group of patients. But vitamin D levels wasassociate with the prognosis of the advanced lung adenocarcinoma，the patientswith vitamin D levels≥20ng/ml have better prognosis.Conclusions：1. Plasma vitamin D levels have no correlation with theprognosis of advanced NSCLC patients.2. In the stratified analysis, plasma vitamin D levels is associated with theOS of advanced lung adenocarcinoma pantients, the patients with vitamin Dlevels≥20ng/ml have better prognosis.3. Both age and clinical stage are independent prognostic risk factors ofPFS in the advanced NSCLC patients. Pathological type and multi-linetreatment are independent prognostic risk factors of OS in the advanced NSCLCpatients.