The Effect Of Propofol And Etomidate On Cellular Apoptosis And Serum Endotoxin During Ischemia-reperfusion Injury Of Rat Intestine

Objective:To compare the effect of propofol and etomidate on cellular apoptosis and serum endotoxin during the ischemia-reperfusion injury of rat intestine and preliminary explore the possible protection mechanism of propofol and etomidate,thus providing the references for drug selection in the practice of clinical anesthesia in future.Methods:A hundred healthy adult male SD rats,weighing 280-330g,were divided randomly into four groups as followsmormal control group(group A,n=10),intestinal ischemia-reperfusion group(group B), propofol group(group C) and etomidate group(goup D),all fasting and water-deprivation for 12 hours before experiment.Group A was executed directly with the absence of any intervention.Group B.group C and group D were given intraperitoneal injection of 1% pentobarbital sodium(45mg/kg)and systematic anticoagulation with heparin(2mg/kg) after the anesthesia effect..After clipping SMA for an hour,group B,C,D received reperfusion of SMA for two hours and micromedicine infusion pump of 0.9%normal saline(10ml/kg/h) with inferior vena cava.Five minutes before reperfusion,normal saline was changed into propofol(10ml/kg/h) for group C and etomidate(0.2ml/kg/h) for group D.After the model was established,the rats were fed after anesthetic awareness and executed at 24h,48h,72h respectively after operation.Then venous blood was collected to test serum endotoxin and ileocecal intestine was incised to detect the histological change and cellular apoptosis of intestinal mucosa.Results:1.normal control group:intestinal villus cells were orderly arranged,regularly intact and morphologically. normal.The single-layer cylindrical cell and lamellar cell of intestinal mucosa aligned normally.2.Intestinal ischemia-reperfusion group:the space of intestinal villus cells enlarged,cells distinctly swelling and destruction, scattered cells dissociated around and space enlargement phenomenon presenced beween submucosa cells and partial cells in the middle. Cytomorphosis and destruction were significantly severe.3.Propofol group cellular edema of intestinal villus cells was not obvious.partial cells suffered destruction and tiny spaces existed in cellular arrangement and histological linkage,which was obviously alleviated compared to group B.4. Etomidate group:the connection beween intestinal villi was comparatively close compared to group B. Cellular edema was not evident, partial cells were subjected to destruction and cellular arrangement was comparatively intact with some destructions and spaces.5.Cellular apoptosis and serum endotoxin of group C and D were lower than that of group B.Serum endotoxin in group D was lower than that in group C (P>0.05),which was not statistically different beween group C and D.Conclusion:1.Cells and tissues of intestinal mucosa undergo injurys and changes with the extent of cellular apoptosis enlarged and serum endotoxin improved.2.Mucosal cell injury resulted from ischemia-reperfusion of intestine is alleviated in propofol group and etomidate group. The possible protection mechanism of propofol and etomidate may lighten or even suppress mucosal cell injury and cellular apoptosis resulted from ischemia-reperfusion injury.