Protective Effects Of Propofol On Intestinal Ischemia And Reperfusion Injury In Rats

Objective To set up a rat model of intestinal ischemia and reperfusion(I/R).To evaluate the changes of intestinal fatty acid binding protein (I-FABP),and Malondialdehyde(MDA),and the morphological changes of intestinal mucosa To investigate the effect of propofol on gut injury following intestinal ischemia and reperfusion,and to provide the basic experimental parameters and evidences.Methods Thirty major adult male Sprague-Dawley(SD) rats (weighting250～300g) were randomly divided into 3(n=10 each):control group(C); Ischemia and Reperfusion group (I/R);propofol group(R).A rat model of intestinal ischemia and perfusion was stablished by blocking the superior mesenteric artery(SMA) with clap for 60 min followed by 60 min reperfusion. Group C rats underwent only midline laparotomy without blocking superior mesemteric artery.Group I/R and R rats underwent a midline laparotomy and then SMA isolation (with a microvascular clip).The SMA occlusion lasted for 60 minutes followed by 60 minutes of reperfusion.Group R rats underwent a additional administration of propofol(10mg/kg) 10min before ischemia and followed by continuous infusion at 10mg/(kg·h). Blood samples were collected from the inferior vena cava prior to ligation,15,30,60 minutes after ligation and 30,60 minutes after reperfusion for determination of I-FABP.The animals were all killed at the end of 1h reperfusion and a small segment of ileum was taken for determination of MDA.The ileum slices were made and pathological changes were also observed under microscope in each group.Results In group C,the level of I-FABP had no significant difference and keep at a low level during the operation time.In group I/R and group R the level of I-FABP increased 15 minutes after ligation and peaked at 30 minutes.It dropped after 30 minutes of reperfusion.But at 60 minutes after I/R injury,it was number of times of the nomlal serum level of I-FABP.Compare with group I/R,the I-FABP in group R decreased during the period of ligation and reperfusion and there was significant difference between the Group I/R and R (P<0.01).The MDA concentrations in group I/R and group R were significantly higher than those in group C (P<0.01) during the period of ligation and reperfusion.The MDA concentrations in group R were lower than those in group I/R (P<0.05).The morphological changes of intestinal tissues were observed with light microscopy.With the staining of HE, In group C,it cannot be seen abnormality in small intestinal tissue.In group I/R the morphological changes of intestinal mucosa could be observed.The mucous layer appeared necrotic,some intestinal mucosal cells shed to enteric cavity,and submucous layer had hyperemia and edema obviously.In group R the necrosis of intestinal mucosa was ameliorated and damage was not obvious as compared with the group I/R.Conclusion Administration with propofo1 can reduce the level of I-FABPand MDA and attenuate the injury of intestinal mucosa in rats after intestinal ischemia and reperfusion injury.Administration with propofo1 can provide significant protection against intestinal ischemia and reperfusion injury,which may be attributed to the ameliorative blood circulation effect and antioxidant effect of propofo1.