The Detection Of Atomic Fluorescence Spectrometry In The Determination Of Tin&arsenic Element For Haemodialysis Water

Haemodialysis water is used for hematodialysis and dialyzate preparation and its quality is closely related to therapeutic effect, and thus the hygienic condition has become a focus of concern. For a laboratory staff of basic center of disease prevention and control, tens to hundreds of haemodialysis water samples need to be detected each year by using the recommendation method of YY 0572-2005 Water for haemodialysis and related therapies(ISO13959:2002,MOD). This method adopts graphite furnace atomic absorption spectrometry for the determination of tin and hydride atomic absorption spectrometry for arsenic. In practice, the above-mentioned method leads to a non-ideal result when tin is detected due to the limitation of numerous factors of influence, and is seldom used for the detection of arsenic because the hydride generation system and disassembling of atomizer are required, moreover, its sensitivity could not reach the standard limit. Therefore, it is very urgent and important to establish a fast, sensitive and accurate detection method. Now Hydride generation-atomic fluorescence spectrometry(HG-AFS) has becomes the preferred method to analyze the elements easy to generate hydride, and been included in national standard method in many domains such as food and drinking water.The study focused on the determination of tin or arsenic and tin in haemodialysis water by HG-AFS. The effects of experimental conditions including instrumental condition, acidity, etc on the atomic fluorescence intensity of arsenic and tin were investigated and optimized. Under the optimized conditions, the working curve of tin showed a linear range from 0 to 10 ng/mL with a correlation coefficient of 0.9993 and a detection limit of 0.089 ng/mL. Whileas the working curve of arsenic displayed a linear range from 0 to 10 ng/mL with a correlation coefficient of 0.9997 and a detection limit of 0.0098 μg/L. The sensitivity is improved 5 times at least compared with other methods.The study indicated that the determination of tin was controlled by a strict medium acidity which could receive a high sensitivity with 1.0percent acidity and 1% KBH4, while the acidity limitation for arsenic was loose. Through continuously testing the mixing standard samples, it showed the method is reliable. Through analyzing the recovery rate, RSD, etc, it showed the method is feasible.This study adopted the HG-AFS method to simultaneously determine tin and arsenic in haemodialysis water. The expetimental results showed that the method is not only simple, sensitive, accurate and little interference, but also can shorten analytical time, improve efficiency, reduce training burden, and save reagents and costs. Therefore, it is suitable for the analysis of tin and arsenic in haemodialysis water in the basic laboratories, and could provide reliable technical support for monitoring of the relevant government department.