The Experiment Research About The Simiao Tongfeng Decoction’s Influence On The Acute Gouty Arthritis Model In Rats Of IL-1β,iL-8 And TNF-α

Objective:Through the establishment of ankle acute gouty arthritis model in rats,to explore the Simiao Tongfeng Decoction’s influence on the acute gouty arthritis model in rats’ synovial of the ankle of IL-1β,IL-8 and TNF-α,discuss the prevention and cure function of the Simiao Tongfeng Deoction to the gouty arthritis,provide the feasibility basis for the application of clinical.Methods: This experiment selects 48 healthy male rats,divided into four groups by blank group,model group,colchicine group and Simiao Tongfeng Decoction group,12 in each group.To make the model for model group,colchicine group and Simiao Tongfeng Decoction group.Colchicine group given colchicine suspension liquid, 1mg·kg-1every time 1, to fill the stomach.Simiao Tongfeng Decoction group given Simiao Tongfeng Decoction liquid, 1mg·kg-1every time 1, to fill the stomach.blank group and model group given isodose physiological saline to fill the stomach.The above four groups are lavage 1 times a day until 7 days.To observe the weight and ankle swelling in rats everyday.Observation of ankle tissue of rats after demineralization,slice.And 100 mg of articular synovial tissue homogenate, centrifugal,Take the supernatant.The determination of IL-1β,IL-8 and TNF-αand statistical analysis.Results: 1.Simiao Tongfeng Decoction group rats activity is normal, normal eating into the water, weight gain, defecate is normal, fur color is good, right ankle swelling, alleviate, activities well.2.Inflammatory factor index results: the model group, IL-1β,IL-8,TNF-α value compared with the blank group were significantly higher(p < 0.01), more have a statistically significant difference between; Simiao Tongfeng Decoction IL-1β,IL-8,TNF-α value compared with the blank group were higher(p < 0.05), but compared with the model group were significantly lower(p < 0.05), statistically significant, Simiao Tongfeng Decoction group with colchicine group is no statistical difference(p < 0.05).3.Histopathological results: blank group, the organization developed normally, fibrous tissue form rule, uniform density. Model group: diffuse inflammation cells infiltration, interstitial edema of osteoporosis, expand capillary hyperemia, visible synovial cell hyperplasia. Colchicine group: organizations have no obvious inflammation effusion cells, complete structure, loss, and capillaries no obvious congestion, edema, fibrous tissue arranged orderly. Simiao Tongfeng Decoction group: no obvious inflammation effusion between organizations, tissue edema, relieve, capillary hyperemia, fibrous tissue returns to normal.Conclusion:1. the Simiao Tongfeng Decoction by xie turbidity detoxifying, promoting blood circulation to remove blood stasis, to improve joint function, reduce joint pain, improve the quality of life, the purpose of the curative effect is remarkable. 2.the Simiao Tongfeng Decoction group in ankle joint synovial inflammation factor IL-1β,IL-8,TNF-α has certain inhibitory effect of regulation, and side effects.