| Objective:Through the establishment of the left coronary artery ligation in rats caused by myocardial infarction after prolonged hypotension animal model. To explore the long-term effects of hypotension on the cardiovascular system damage and invigorating qi and ascending depression party and party in pure invigorating qi and ascending the influence of depression with traditional Chinese medicine Huoxue Huayu on the animal nodel of blood pressure, blood clotting function, cardiac function and left ventricular structure and myocardial collagen, and compare the index for the strength of effect size, clarify Yiqi Shengxian Decoction and its disassembled Decoction in the treatment of myocardial infarction after prolonged hypotension state mechanism.Methods:1 build model to measure the normal blood pressure of rats before and after ligation of left coronary artery of rats, after two weeks of rats left ventricular ejection fraction (LVEF) and tail arterial blood pressure (one, two) the same experiment.2 Experiment one group, LVEF value 50% or less, tail artery systolic blood pressure> 90 mmHg in heart failure rats were randomly divided into nodel group, LVEF value 50% or less, tail artery systolic blood pressure of 90 mmHg or less hypotension after rats were randomly divided into heart failure group, setting up control (not only in the process of building the threading ligation rat). Deionized water intervention in eight weeks, each rat measuring blood pressure once a week.3 Experimental two group, LVEF value 50% or less, tail artery systolic blood pressure of 90 mmHg or less rats were randomly divided into 4 groups, respectively is hypotension after heart failure group, yiqi liters in the treatment group, yiqi liters in treatment group (treatment group and promoting blood circulation to remove blood stasis drugs. Adopt corresponding lavage treatment of Chinese medicine, drug intervention in jight weeks, each rat measuring blood pressure once a week.4 intervention after 8 weeks, echocardiography, for each group based in rats, to detect rats blood coagulation function, observe the general histology of the heart (HE staining), to detect myocardial I, III collagen (immunohistochemistry), using real-time quantitative PCR to detect nyocardial I, III collagen mRNA levels (one, two) the same experiment.Results:Experiment 1 results1.1 echocardiography test resultsHeart failure after a low blood pressure, heart failure model group compared with control group, ventricular septal end systolic thickness, the thickness of the ventricular septal end-diastolic thinner (P<0.05), left ventricular ejection fraction values were lower (P<0.05), left ventricular end systolic diameter and end-diastolic diameter increased (P<0.05); Hypotension after heart failure group compared with control group, after eft ventricular end systolic wall thickness, left ventricular posterior wall thickness of the end-diastolic thinner (P<0.05); Heart failure after the comparison of the model control group, after left ventricular end systolic wall thickness, left ventricular end-diastolic wall thickness after no significant change (P>0.05).Hypotension after heart failure group compared with model group hf, ventricular septal end systolic thickness, after the interventricular septum, eft ventricular end-diastolic thickness thinning of the wall thickness of the end-diastolic (P<0.05), left ventricular end systolic diameter and end-diastolic diameter increased (P<0.05), left ventricular ejection fraction, left ventricular posterior wall thickness of end systolic no significant change (P>0.05).1.2 the rat tail artery blood pressure (systolic pressure) test resultsIntervention during 1 to 8 weeks, every week to measure blood pressure of the situation is as follows:heart failure after low blood pressure, heart ailure model group and control group comparison, hypotension after heart failure group blood pressure value was lower than those of heart failure of model group (P<0.05), heart failure model group blood pressure value is low in the control group (P<0.05).1.3 the rat blood coagulation index test resultsHeart failure after a low blood pressure, heart failure model group and control group, the rats of prothrombin time (PT) and the ratio (INR), PT PT international standardization percentage activity%(PT), thrombin time (TT) no significant statistical difference (P>0.05); Heart failure model group compared with control group, the rats of fibrinogen concentration (dFbg) increased (P<0.05), low blood pressure after heart failure group compared with control group, the rats of the part activated clotting time (APTT) live enzymes shortened (P<0.05); Heart failure model group compared with control group, the rats of the part activated clotting time (APTT) live enzymes shortened (P<0.05).1.4 myocardial Ⅰ,Ⅲ collagen type test results Conclusion by immunohistochemistry and rt-pcr detection, hypotension after heart failure group compared with model group hf, hypotension after heart failure group of myocardial Ⅰ,Ⅲ collagen content were higher than in heart failure model group (P<0.05); Heart failure after a low blood pressure, heart failure model group compare, respectively, compared with control group, the myocardial Ⅰ and Ⅲ collagen content were higher than in control group (P<0.05).Experiment 2 results2.1 echocardiography test results Yiqi liters in the treatment group, yiqi liters in treatment group, promoting blood circulation to remove blood stasis drugs treatment group compared with hypotension group after heart failure, ventricular septal end systolic thickness, ventricular septal thickening of end-diastolic thickness (P<0.05), left ventricular end systolic diameter, left ventricular end-diastolic diameter increased (P<0.05), left ventricular end systolic wall thickness, left ventricular end-diastolic wall thickness after no significant change (P>0.05).Yiqi liters in the treatment group, yiqi liters in treatment group, promoting blood circulation to remove blood stasis drugs, the treatment group to compare the ventricular end-diastolic insulation thickness between the comparison:drug treatment group compared with yiqi liters for activating blood circulation in the whole party thickening of the treatment group (P<0.05). Left ventricular end systolic wall thickness after comparison:yiqi in drug treatment group compared with yiqi litres in the whole party thickening of the treatment group (P<0.05). The comparison of eft ventricular end systolic diameter:yiqi liters in the whole party narrowing of the treatment group with promoting blood circulation to remove blood stasis drugs treatment group (P<0.05). The comparison of left ventricular ejection fraction value:yiqi liters in whole party treatment group compared with yiqi rise in the value of the high drug treatment group (P<0.05), yiqi liters in drug treatment group with promoting blood circulation to remove blood stasis drugs high value of the treatment group (P<0.05). The chamber between the end systolic insulation thickness, left ventricular end-diastolic wall thickness after compared with no significant change (P>0.05).2.2 the rat tail artery blood pressure (systolic pressure) test resultsDrug intervention for 8 weeks, yiqi liters in the treatment group, yiqi liters in treatment group, promoting blood circulation to remove blood stasis drugs treatment group, respectively, compared with hypotension after heart failure group, yiqi liters in the treatment group, yiqi liters in treatment group, promoting blood circulation to remove blood stasis drugs treatment group’s blood pressure values from heart failure after hypotension group (P<0.05), high blood pressure value, yiqi liters in the whole party blood pressure value of the treatment group with yiqi liters in drug therapy group (P<0.05), high blood pressure value, yiqi liters in treatment group )lood pressure value is promoting blood circulation to remove blood stasis drugs high blood pressure value of the treatment group (P<0.05).3.3 the rat blood coagulation index test resultsYiqi liters in the treatment group, yiqi liters in treatment group, promoting blood circulation to remove blood stasis drugs treatment group compared with hypotension after heart failure group, fibrinogen concentration in rats (dFbg) no significant statistical difference (P>0.05); Heart failure after a low blood pressure and promoting blood circulation to remove blood stasis drugs treatment group comparison, rats of prothrombin time (PT) shortened (P<0.05); Hypotension after heart failure group respectively with yiqi liters in the treatment group, yiqi liters in treatment group, promoting blood circulation to remove blood stasis drugs treatment group, compared to PT percentage activity (PT%) increased (P<0.05); Drug treatment group respectively for activating blood circulation and yiqi liters in the treatment group, compared hypotension after heart failure group, PT international standardization ratio (INR) increased (P<0.05); Hypotension after heart failure group and yiqi liters in the treatment group, yiqi liters in drug treatment group compared with thrombin time (TT) were shortened (P<0.05); Yiqi liters in the whole party treatment group compared with promoting blood circulation to remove blood stasis drugs treatment group, thrombin time (TT) extension (P<0.05); Hypotension after heart failure group and yiqi liters in drug treatment group compared with part activated slotting time (APTT) live enzymes shortened (P<0.05).4.4 myocardial I, III collagen type test resultsConclusion by immunohistochemistry and rt-pcr detection, yiqi liters in he treatment group, yiqi liters in treatment group, promoting blood circulation to remove blood stasis drugs treatment group compared with hypotension after heart failure group, myocardial I, III collagen content were lower (P<0.05); Yiqi liters in the whole party treatment group espectively with yiqi liters in treatment group, promoting blood circulation to remove blood stasis drugs treatment group comparison, myocardial I, III collagen raRNA levels were lower (P<0.05); Yiqi liters in the treatment group with promoting blood circulation to remove blood stasis drugs treatment group comparison, myocardial I, III collagen mRNA were decreased (P<0.05).Conclusion:1 Heart failure after myocardial infarction rats existence state of low blood pressure, heart failure after myocardial infarction in the rat of "nyocardial fibrosis degree aggravating, left ventricular end systolic diameter and increased end-diastolic diameter, left ventricular end systolic .vail thickness, left ventricular end-diastolic wall thickness thinning, left ventricular ejection fraction value decreased, blood pressure drops, disordered arrangement of myocardial cells, myocardial collagen type Ⅰ,Ⅲ, a large amount of deposit, the relative content of myocardial Ⅰ, Ⅲ collagen mRNA increased.2 State of hypotension in rats with heart failure after myocardial infarction in rats of left ventricular ejection fraction values were positively correlated.3 Yiqi liters in the treatment group, yiqi liters in drug therapy group, promoting blood circulation to remove blood stasis drugs can improve heart failure after myocardial infarction rats state of low blood pressure, reduce the degree of myocardial fibrosis rats with heart failure after myocardial infarction, reduced left ventricular end systolic diameter and end-diastolic diameter, higher blood pressure and left ventricular ejection fraction value, improve the rat myocardial histological appearance commonly, reduce the deposition of myocardial collagen type Ⅰ,Ⅲ, reduce the relative content of myocardial I, III collagen. With yiqi liters in the whole party the strength of the effect of treatment group is yiqi liters in drug therapy group, promoting plood circulation to remove blood stasis drugs, drugs for activating blood circulation group strength is small. |
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