Studies On Ginseng Fruit Saponins Proliposomes

Ginseng fruit Araliaceae ginseng PanaxginsengC.A.Mey.,which is also a kind of precious medicines.Ginseng fruit has the effect of a variety of diseases prevention and strong tonic effect on the human bodyin,and in our country it has a long history of medicinal.Because of its rich ginsenosides and significant physical activity,Ginseng fruit has attracted wide attention and research of scholars at home and abroad[2,3].Ginseng fruit contains a variety of chemical composition, the main component of ginsenosides, polysaccharides, alkaloids, volatile oil, amino acids, inorganic elements and sterols.It has been reported that the oral biovailability of Ginseng fruit saponins is very low and the residence time of ginsenosides in the circulation is short.In order to improve the bioavailability of Ginseng fruit saponins, to extend the preservation time and to make it possible to transfer it into other dosage forms, we made some Ginseng fruit saponins liposome. For the purpose of improving the ginseng fruit saponins oral bioavailability,we use production feasible ethanol injection method and spray drying method to complete the preparation of GFS proliposome.single factor test was used to examine the influencing factors that will affect the average size and the encapsulation efficiencyof liposome; and the optimal preparation conditions as well as prescription parameters are found out through response surface optimization scheme.And we studied the basic physical and chemical properties and preliminary stability of GFS proliposoes prepared by spray drying.At the same time we made some liposome suspension of Ginseng fruit saponins to detect the particle size, encapsulation efficiency and preliminary stability of the liposome. The results show that the average size of Ginseng fruit saponins liposome is 236.1 nm, and the encapsulation efficiency of the Ginseng fruit saponins liposome is 68.2%,which have both met the requirements of the Chinese Pharmacopoeia[1].The ethanol injection method to prepare the liposome is simple, stable, reproducible and suitable for large scale production.This paper has established HPLC method for determination of GFS liposomal drug content,separated by dialysis liposomes and free drug,established method for the determination of encapsulation efficiency,established in vitro release detection method of GFS precursor liposomes.In vivo pharmacokinetics and preliminary safety studies evaluate the advantages of GFSP’S agent.In this paper, pharmaceutical raw materials and Zhenyuanpian respectively as the control drug and the positive control drug,developed in this laboratory of GFS proliposomal agents, on the pharmacokinetics of oral gavage in rats were studied.Experimental results show that,the proliposomes’Aucoâ†'t was 2082.492±408.33 μg · h · mL-1,the pills’ Auc0â†'t was 1151.38±198.29μg. h mL-1,the control drugs’Aucoâ†'t was 733.32±113.82 pg·h·mL-1.The paramaters showed a significant difference(P<0.01).The relative bioavailability were 180.89% and 283.91% respectively.Other parameters had no significant difference(P>0.05).