Ginseng Saponins Rg3through To HDAC3Influence Melanoma Cell Proliferation And Apoptosis

Objective:Ginseng is a valuable tradition Chinese medicine in China, and its main efficacycomponents is ginseng saponins Rg3is first applied to clinical antitumor transfer CanYicapsule of the main active ingredient of traditional Chinese medicines. InducInductionof apoptosis of tumor cells. Inhibition of tumor cell adhesion, invasion and metastasis,and inhibiting tumor angiogenesis. Lymphatic channel transfer resistance. Reversemultidrug resistance in tumor cells and so on. Attenuated synergies with thecombination of effective resistance to a variety of action such as metastasis tumorproliferation apoptosis.Epigenetics include DNA methylation, histone modification, chromatin remodeling,RNA interference, etc. Occupies an important position in the covalent modification ofhistone, covalent modification of histone: ubiquitin, phosphorylation, acetylation,methylation, etc., they can be alone or together regulate gene transcription, used in playan important in the development of tumor. Histone acetylation modification includingacetyl groups and to acetyl respectively by acetyl transferase (histone acetyltransferases,HATs) and histone acetylation enzyme (histone deacetylases, HDACs) to regulateHDACs acetylation of different types of nuclear transcription factors and protein, etc.,and inhibit the expression of multiple tumor suppressor protein and closely associatedwith a variety of oncogenes, causes cell over proliferation and tumorigenesis. Thisexperiment adopts the melanoma cell line cells mescl-28, application of ginsengsaponins mescl Rg3observation-28HDAC3proliferation and apoptosis detection ofchange. II．MethodsIn vitro mescl-28people melanoma cells, the experiment is divided into four groups(control group, and25mu mol/lRg3,50mu mol/lRg3,100mu of mol/lRg3) Rg3CCK8method impact on cell apoptosis; Inverted microscope observation of cell morphologyunder different concentrations of Rg3; HDAC3changes under different concentrationsof Rg3TRITC method; WesternBlot method detecting antigen (PCNA) and cellproliferation HDAC3, Bcl-2and apoptosis protein expression of Caspase-3; RT-PCR to detect the expression of PCNA and HDAC3.III． Results1. Detection of CCK8Rg3for cell growth inhibition,24hours,48hours,72hours50mol/L.Rg3of cell growth inhibition rate were18.4%,23%,27%.2. Inverted microscope to observe the cell growth conditions under differentconcentrations. Control cells stick wall of good, full, angular into irregularpolygons.Rg3group cells slightly with the increase of concentration, it becomemorphology change, gradually less quantity,high concentration of cell debris can beobserbed.3. Detection of TRITC hair HDAC3under different concentration change, obviouslywith the increase of concentration of Rg3, HDAC3gradually less.4. Western Blot method, the change of PCNA, obviously with the increase ofconcentration of Rg3, HDAC3gradually reduce, PCNA was gradually reduced. The Bcl-2and Caspase3increase gradually.5.RT-PCR detection HDAC3and PCNA. Visible HDAC3and PCNA was graduallyredce with the increase of concentration of Rg3.IV.ConclusionsGinseng saponins Rg3inhibit the growth of melanoma cells mescl-28, ginsengsaponins Rg3inhibit HDAC3expression. Ginseng saponins Rg3into cell apoptosis...