Experimental Research Of Disobulbin B Growth Inhibition And Promoting Apoptosis On SGC-7901 Cells

Gastric cancer is one of common malignant tumors in worldwide..In 2005, gastric cancer became the leading cause of death from cancer in our country.Duanjiju’s report shows that According to the China health statistics annual report the annual mortality rate of gastric cancer in China was 30.80/10 million, which was more than 2 times of the world average.Gastric cancer has become a major disease that threatens the people’s health. At present the major treatment of gastric cancer is surgical operation. But the effect is not good.In our country,the Traditional Chinese Medicines plays an important role in treating gastric cancer.Accumulated studies have confirmed that Chinese herbal compounds may inhibit proliferation,induce apoptosis,inhibit metastasis and telomerase activation,regulate immune function and reverse drug-resistance in gastric cancer.Disobulbin B is the tuber of potato plants Dioscorea bulbifera 1. It is cold and bitter taste,and it has the role of cooling blood,eliminating gall,sending fire and detoxification.In order to study the Disobulbin B anti-cancer mechanism,this study proposed by observing the effect of Disobulbin B on cultured in vitro and the Effects on cell cycle protein cyclin D1 protein and VEGF expression level.And preliminary explore the molecular mechanism of its action.This study of Disobulbin B can provide scientific theoretical and experimental basis for treatment of cancer of the stomach.It is of great significance to promote the traditional Chinese medicine clinical transformation.Objective :To study the effect of Disobulbin B on SGC-7901 cells growth in vitro and its mechanisms. Methods:SGC-7901 Cells were treated with 0~320μg/ml of DB. Cell proliferation was detected by cell counting Kit-8.Cell cycle was detected by flow cytometry.The expression of cyclin D1 and VEGF were detected by Western blot. Cell morphology changes were observed under optical microscope. Results: After incubation with different concentrations of DB for 48 h. SGC-7901 cells proliferation was inhibited dramatically and the highest inhibition rate was 59.8%.The structure of SGC-7901 cells changed greatly.Observing the change of nucleus morphology under thefluorescence microscope after the cells treated with Disobulbin B and stained with DAPI.Conclusion: DB induced an increase of G0/G1 phase cells. The expression of cyclin D1 and VEGF were down-regulated at protein levels. DB exerted this effects in a does-dependent manner. The morphological changes of nuclear were observed under fluorescence microscope. After the cells treated with DB, cell line SGC-7901 cells exhibited remarkable apoptosis characters such as membrane break, chromatin condensation and fragmentation. DB could inhibit migration of SGC-7901 cells.