| Objective Analyzing the basic clinical data and digit ratios of premature ovarian failure,compare reproductive hormone levels,BMI between premature ovarian failure and normal healthy women, exploring the digit ratios distribution of premature ovarian failure, is aim to provide theoretical basis for early diagnosis and treatment of premature ovarian failure.Methods 91 cases of patients was diagnosed as premature ovarian failure in three consecutive times during May 2008- February 2015 in Center of Reproductive Medicine,General Hospital of Ningxia Medical University. Do general physical examination, gynecological examination, reproductive hormones, B ultrasonography, chromosome screening, and records relevant results. There were 91 cases of finger length data. 255 patients of control group treated in the Hospital during the same time were reproductive hormones and B ultrasonography normal healthy women. Digit lengths of the fingers were measured from photocopies of the ventral surface of the hand and by actual finger measurements. To analysis the basic clinical data,compare chromosome screening,BMI, reproductive hormone levels and digit ratios between premature ovarian failure and normal healthy women.Results 1 In this study, 91 patients with premature ovarian failure, aged 29.07± 5.90(18~40);height 162.15±5.14 cm(150~176cm);weight 56.95±8.05 kg(39~82kg);BMI:21.66 ± 2.93 kg /m2(16.53~ 29.76 kg /m2); age at menarche 15.99±6.71(11~20). FSH 60.57±30.64 m IU/ml,LH 40.20±18.34 m IU/ml,E2 40.42±84.31pg/ml,PRL 8.56±6.10ng/ml,T 32.09±18.37ng/ml.ANA and ACA antibody test line in 30 cases, ANA positive in 4 cases,the positive rate of 13.33%; ACA positive in 4 cases,the positive rate of 13.33%;TGAb and TPOAb examination in 31 patients, TGAb positive in 7 cases,the positive rate of 22.58%; TPOAb positive in 7 cases,the positive rate of 22.58%.250 cases in the control group, aged 29.87±4.89(18~43);height 162.47± 4.34cm(150~173cm);weight 58.68±8.76 kg(39.50~95kg);BMI:22.26±3.23 kg /m2(15.82~ 37.58 kg /m2);age at menarche 13.68±1.53(10~19). FSH 5.91±2.04 m IU/ml,LH 4.43±2.63 m IU/ml,E2 53.91±40.89 pg/ml,PRL 14.86±16.89 ng/ml,T 38.31±17.60 ng/ml. ANA and ACA antibody test line in 10 cases,ANA positive in 1 cases,the positive rate of 10%; ACA detect negative results;TGAb and TPOAb examination in 13 patients, TGAb positive in 3 cases,the positive rate of 23.08%; TPOAb positive in 4 cases,the positive rate of 30.77%.2 The two groups,those who exclude chromosomal polymorphism between the two groups BMI、E2、T were not significantly different(t=0.922、1.961、2.467,P>0.05),while FSH、LH、PRL、age of menarche between the two groups the differences were statistically significant(t =-26.440、-28.356、2.809、4.597,P<0.05).3 Premature ovarian failure group and the control group the BMI distribution constitute significant difference(X2=8.089,P<0.05).4 Premature ovarian failure group and the control group about finger digit ratios analysis showed no significant difference(P>0.05)。5 Premature ovarian failure group and the control group 2D:4D ratio distribution constituted, the difference was not statistically significant(X2=0.017,0.042,P>0.05).6 Premature ovarian failure group and the control group obese than non-obese finger digit ratios analysis, was no statistical significance(P>0.05)。7 Premature ovarian failure,6 cases of primary amenorrhea,85 cases of secondary amenorrhea;compare two groups,the right 4D:5D difference was statistically significant(t=-2.073,P<0.05).8 Premature ovarian failure group ANA/ACA positive in 8 cases and negative in 22 cases, compare two groups,there was no statistically significant.(P>0.05).Conclusion 1 Delayed age at menarche early screening may be one of the reference index in patients with premature ovarian failure2 Premature ovarian failure patients with the number of obese and less3 Referring to female fertility may be less relevant digit ratios analysis4 4D:5D may be one indicator of secondary amenorrhea screening of patients with premature ovarian failureObiective Analysising the first exon of AR gene(CAG)n polymorphism and SHBG gene promoter(TAAAA)n polymorphism of premature ovarian failure, polymorphism and digit ratios,is aim to investigate the association between gene polymorphism and premature ovarian failure, gene polymorphism and gene digit ratios.Methods ABI 3730 XL sequencer was used to determine the AR gene(CAG)n and SHBG gene promoter(TAAAA)n polymorphism. Comparing the distribution differences of the first exon of the AR gene(CAG)n polymorphism, SHBG gene promoter(TAAAA)n polymorphism between the premature ovarian failure and normal healthy women group. Comparing digit ratios between short and long repeated groups of the first exon of the AR gene(CAG)n, SHBG gene promoter.Results 1 The mean(CAG)n polymorphism between the premature ovarian failure and normal healthy women group were 22.67±2.87 and 22.62±2.68,the difference was not statistically significant(t=0.194;P=0.846).(CAG)n repetitions with n = 22 as the dividing point,the two group were divided into short repeat polymorphism(n<22) and long repeat polymorphism(n≥22). The short polymorphism of the premature ovarian failure and normal healthy women group were more than the long repeat polymorphism, but the distribution in the two groups was no significant different.(X2=0.112;P=0.738).2 The(TAAAA)n alleles average repetitions between the premature ovarian failure and normal healthy women group were 7.85 ± 1.31 and 7.24 ± 1.60, which has statistically significant(t =4.014, P = 0.000). Refer to the domestic literature,when 8 was as the dividing point,the two groups were divided into short and long repeat polymorphism,the distribution of(TAAAA)n polymorphism in the premature ovarian failure and normal healthy women group have significant difference(X2=3.849;P=0.05). Refer to foreign literature, when 8 was as the dividing point, the distribution of(TAAAA)n polymorphism in the premature ovarian failure and normal healthy women group also have significant difference(X2=5.342;P = 0.021).3 Premature ovarian failure group and the AR gene(CAG) n left and right of each digit ratio difference was not statistically significant(t=0.010,-1.872,0.719,-0.797,0.803,-0.432,0.944,0.973,1.892,0.593,0.399,0.099,P>0.05). Control and AR gene(CAG) n left and right of each digit ratio relatively left 2D: 5D, 3D: 5D, 4D: 5D difference was statistically significant(t = 2.420,2.875,3.657, P <0.05), the rest of the digit ratio no difference(t=-0.784,1.437,-0.848,-0.034,1.170,0.145,-1.538,0.561,1.597, P>0.05).4 To national standards, premature ovarian failure group and SHBG gene(TAAAA) n repeat left and right of each digit ratio 4D:5D difference was statistically significant(t = 2.118, P <0.05), the rest of the digit ratio no difference(t =-0.012,0.369,-1.385,-0.478,0.843,0.677,-1.549,-0.937,0.973,0.605,1.321, P> 0.05). Control and SHBG gene(TAAAA) n repeat length polymorphism groups around long fingers no statistically significant difference compared(t=-1.567,0.258,-0.926,0.354,-0.251,-0.049,1.467,0.339,1.309,- 0.189,0.368,0.452, P> 0.05).With foreign standards, premature ovarian failure group and SHBG gene(TAAAA) n repeat length polymorphism group about finger length ratio difference was not statistically significant(t =-0.242,-0.434,-0.786,-0.209,-1.203,-0.899,-0.761,0.149,-1.165,-0.774,-0.881,-1.010, P> 0.05). Control and SHBG gene(TAAAA) n repeat length polymorphism group about finger length ratio difference was not statistically significant(t = 1.240, 0.350, 0.931, 1.183, 0.644, 1.310,-0.539, 1.509,-0.363, 1.332, 0.112, 0.630, P> 0.05).5 To national standards, premature ovarian failure group AR gene(CAG) n, SHBG gene(TAAAA) n repeat polymorphism group about finger length ratio showed no difference( F=0.077,1.989,1.273,0.463,0.710,1.038,1.417,0.609,2.164,2.519,2.565, P > 0.05). In control group AR gene(CAG) n, SHBG gene(TAAAA) n repeat polymorphism groups around the left hand finger relatively longer than the 2D: 5D, 3D: 5D, 4D: 5D difference was statistically significant(F=2.289,3.635,4.693,P<0.05), the rest of the digit ratio no difference(F=0.986,0.997,0.417,0.043,0.411,0.865,1.062,0.105,0.810, P>0.05).With foreign standards, premature ovarian failure group AR gene(CAG) n, SHBG gene(TAAAA) n repeat polymorphism finger is longer than the comparison group about the right 2D: 5D, 3D: 5D,4D: 5D difference was statistically significant(F=3.833,3.041,2.577,P<0.05), the rest of the digit ratio no difference(F=0.092, 1.619, 0.263, 1.217, 0.844, 0.214, 0.539, 0.711, 0.579,P>0.05). In control group AR gene(CAG) n, SHBG gene(TAAAA) n repeat polymorphism group about the left 2D: 5D, 3D: 5D,4D: 5D(F=2.861,3.307,5.924,P<0.05), the rest of the digit ratio no difference(F=0.556, 0.702, 0.292, 0.124, 1.105, 0.226, 0.847, 0.807, 1.716,P>0.05).Conclusion 1 In premature ovarian failure AR gene(CAG) n repeat length no difference with other groups2 In premature ovarian failure SHBG gene(TAAAA) n repeat length polymorphism and various other groups3 Left hand 2D: 5D, 3D: 5D, 4D: 5D may(CAG) n repeat polymorphism correlated4 Premature ovarian failure left 4D: 5D may(TAAAA) n repeat polymorphism5 5 finger too short may affect(CAG) n,(TAAAA) n repeat polymorphism... |
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