| 1 In this study, macroporous resin adsorption-silica gel column-C18 reverse phase medium pressure column and semi-preparative liquid relative ginseng oleanolic acid type ginsenosides Ro were isolated and purified. This method is simple, convenient, low-cost and relatively stable, suitable for ginseng ginsenosides Ro separation, purification, can be obtained in a short time a lot of fast monomer ginsenosides Ro, future researchers can follow the ginsenoside Ro the method can provide a large number of studies to obtain a monomer compound.2 Ginsenoside Ro is one of the high-abundance saponin in ginseng, which is the most widely used dietary supplementary, and has many multiple health promotion effects. However, knowledge about metabolism of ginsenoside Ro is very few. In order for profiling and identification metabolites of ginsenoside Ro, the identification of metabolites of ginsenoside Ro in both feces and urine using a high performance liquid chromatography tandem mass spectrometry(HPLC MS/MS) was described in this job. Quantitative analyses of six of the most abundant metabolite were also performed. There were finally found 14 metabolites, besides the parent drug. Among these metabolites, zingibroside R1, chikusetsusaponin IVa, silphioside F, glucosyl oleanolate and oleanolic acid were originated from deglycoslation; vitalboside A, acutoside A and asteryunnanoside F were transformed from glycoslation of oleanolic acid; glucosyl oleanonate and oleanonic acid were produced by oxidation; 11-oxoerythrodiol, 15-hydroxy-3-oxo-olean-12-en-28-oic acid, 15-hydroxy-olean-12-en-28-oic acid and morolic acid should be produced through multiple reactions. Total recovery of metabolites over 72 h was approximately 60%. Hydrolysis of the glycosidic bond on the aglycone was the main pathway of ginsenoside Ro. According to quantitative results show that, in feces, there was approximately 10% of ginsenoside Ro was observed to be excreted in the form of zingibroside R1, glucosyl oleanolate and ginsenoside Ro respectively. There was 25.9% of ginsenoside Ro was detected to be excreted in the form of oleanolic acid. Other metabolites were observed to be in relatively low abundance. There was approximately 0.12% of ginsenoside Ro was observed to be excreted in the urine.3 Comparative the species of oleanolic acid type saponins and the amount of ginsenoside Ro in roots, stems and leaves of ginseng and American ginseng. Observe the distribution characteristics in both. Identification and quantitative analysis by high performance liquid chromatography tandem mass spectrometry. Column: Agilent TC-C18(4.6 mm×250 mm,5 um); The mobile phase consisted of two eluents: Acetonitrile and water with 2.5 mmol/L Ammonium acetate(w/v) and 0.05‰ Ammonia(v/v); Gradient elution; The flow rate was 1 mL/min; 203 nm as detection wavelength. Six compounds of oleanolic acid type saponins were identified. They were ginsenoside Ro(1), chikusetsusaponin Iva(2), zingibroside R1(3), stipulenaoside R2(4), arrnatoslde(5) and elatoside K(6). The compound 1, 2 and 3 were all found in root, stem and leaf of ginseng. About American ginseng, compound 1, 3 and 4 were found in stem; compound 1 and 4 were in leaf. And above all six compounds were in root. Root as a main storage part of ginsenoside Ro and the amounts in root of American ginseng(0.576 %) was more than the root of ginseng(0.214 %). The amounts of ginsenosides Ro between different parts in both were similar, they were all root> stem> leaf. The Species of oleanolic acid type saponins were differences in different parts of Panax ginseng and American ginseng. But the distribution of ginsenoside Ro was similar to different parts in both. The roots as the main part of accumulation of oleanolic acid type saponins. |
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