| The studies used the routine methods and HPLC chromatogram to identify the medicinal materials of Mutong in different sources. At the same time, the technics of extraction and hydrolyzation and the mensuration of HPLC for the target component were studied, and the contents of the target component of Mutong coming from different producing areas, different sources and different part of plant were mensurated. The results were as follow:1. Compared with traditional extraction, ultrasonic method could save time, was easy to operate, improved extraction rates and did not need heating. The order of factors to affect the Oleanolic Acid extraction was extraction times>ethanol concentration ultrasonic power>ration of interval time to ultrasonic time. The optimum ultrasonic extractions were: ethanol of 50%, ultrasonic power of 1200W, time of 30 min, ration of interval time to ultrasonic time of 0.5 to 1.2. Applying D101 macroreticular resin to absorb and purify akeboside was feasible, and the akeboside was in 50% ethanol mainly. The content of adsorption was 2.96mg · g-1 , the elutive ration of akeboside was above 90%, the purity reached 51.3%, extent of refine was 169.3%.3. Akeboside had been hydrolyzed by the different concentration of H2SO4 for the same time, and the content of oleanlic acid was mensurated. The result was that the content of oleanlic acid in the sample hydrolyzed by 1.5 mol·L-1 and 2.0 mol·L-1 H2SO4 was the highest of all. The study of hydrolyzation for different time found which hydrolyzed for 4h was suitable.4. The study for method which HPLC mensurated the content of oleanlic acid indicated that Oleanolic acid was sperated on a Hypersil ODS column. The mobile phase was methanol-water. The flow rate was 0.8mL·min-1 with linear gradient .UV detection wavelength was 215nm. Column temperature was 20℃. Under these conditions, oleanolic acid in Mutong was separated well. The relationship of injection amounts and peak was linear(r=0.999 9) in the range of 1.226.10ug and the recovery rate was 98.8%. It shew that the method was simple, accurate and could be used for the quality study of Mutong.5. The content of the target component was different in different plant of source. The content of oleanolic acid in Akebia quinata(Thunb.)Decne. was higher than Akebia trifoliate(Thunb.)Koidz., and Chuan Mutong didn't contained oleanolic acid. The content of oleanolic acid changed with the samples coming different producing areas. Akebia trifoliate(Thunb.)Koidz. Coming from Tongjiang, Ya'an, Pingwu and Luding of Sichuan province , Zunyi of Guizhou province contained oleanolic acid lowlier than which coming from Yingjing, Yibing, Qingchuan, Hanyuan of Sichuan province, and Jingmen of Hubei province. Akebia quinata(Thunb.)Decne. coming from Hanyuan, Yingjing, Yibing, Qingchuan of Sichuan province contained oleanolic acid highlier than Ya'an of Sichuan province, and Hanyuan was the highest one. The samples of different plant part contained different content of oleanolic acid. The root of Akebia trifoliate(Thunb.)Koidz. was the highest one, the stem growing for 1 or 2 years was lower, and the content of oleanolic acid in the perennial stem was 5. 6581 mg·g-1.6. HPLC was used to study the fingerprint of Akebia quinata(Thunb.)Decne., Akebia trifoliate(Thunb.)Koidz. and Chan Mutong under these conditions: Hypersil ODS column, with mixtures of water and methanol as mobile phase in a gradient mode. The flow rate was 1 ml/min. The temperature of column was 20℃. The wavelength of detection was 215 tun. The normal models for each medical material were built. The similar degree of the different sample was worked out and compared with each other. The results indicated that the similar degree between the same medical materials of different production areas and between Akebia quinata(Thunb.) Decne. Akebiatrifoliate(Thunb.)Koidz. were high, but it was low as the twos were compared with Chuan Mutong. So these HPLC fingerprints can been used as exclusive fingerprint to identify Mutong medical materials.
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