The Expression And Significance Of CK13 And P63 In Cervical Intraepithelial Neoplasia And Cervical Carcinoma

Objective: By studying the CK13 and P63 expression in cervical intraepithelial neoplasia and cervical carcinoma, this paper explore the CK13 and p63’s appearance,development and their correlations in cervical carcinoma, and further explore its clinical diagnosis and treatment value for cervical intraepithelial neoplasia and cervical cancer.Methods: ①.Selecting clinical cervical biopsy cases, which include 15 cases of normal cervical tissue, 21 cases of I grade cervical intraepithelial neoplasia(CIN I), 20 cases of cervical intraepithelial neoplasia(CIN II), 25 cases of cervical intraepithelial neoplasia(CIN III) and 26 cases of cervical cancer.(According to the International Federation of Gynecology and obstetrics(FIGO,2009) standard, the cervical squamous cell carcinoma groups were divide into two clinical stages and three pathological grades.which including 14 cases in I staging, 12 cases in II staging, 11 cases in well-differentiated group, 8 cases in mid-differentiated group and 7cases in poorly differentiated group.) ②.Detecting CK13 and p63 expression in normal cervical tissue and all levels of cervical intraepithelial neoplasia(CIN) and squamous cell carcinoma of cervix(SCC) by immunohistochemical S-P method.③ Using statistical software SPSS statistics 17.0 to do data Statistics and correlation analysis.Results:①. CK13 expression in different tissues of cervix :CK13 has a high expression in normal cervical tissue, it’s positive rate is 93.33%, but in CINI(cervical intraepithelial neoplasia I grade), CINII(cervical intraepithelial neoplasia II grade), CINIII(cervical intraepithelial neoplasia III grade) and SCC(squamous cell carcinoma of cervix), the expression of CK13 is gradually reduced, it’s positive rate are respectively61.90%,40.00%, 28.00%and26.92%, which is obviously lower than in normal cervical organization. Statistical analysis shows that the expression of CK13 in normal cervical tissue, CINI, CINII, CINIII and SCC exist significant differences(P<0.01), the loss of CK13 expression can be used as a symbol of CINIII and SCC.②.P63 expression in different tissues of cervix:P63 has a low expression in normal cervical tissue, it’s positive rate is 33.33%, but in CINI, CINII, CINIII and SCC, the expression of p63 is gradually increased, it’s positive rate are respectively,47.62%,75.00%,92.00%and92.31%, which is obviously higher than in normal cervical organization. Statistical analysis shows that the expression of p63 shows significant differences(P<0.01) in normal cervical tissue,CINI, CINII, CINIII and SCC, while shows no significant differences in CINIII and SCC(P>0.05). ③. The expression of CK13 is negatively correlated with p63. CK13 has different expression at different stages of cell differentiation.④.The positive expressionrate of CK13 at different clinical stage of squamous cell carcinoma is respectively42.86%( CIN I grade), 8.33%( CIN II grade); While studies have found that expression of different pathological grading of squamous epithelial Carcinoma of cervix will present the same change, its expression rates were: 36.36% for CIN I grade, 25% for CIN II grade, and 14.29% for CIN III grade(P<0.01).The expression of p63 in different clinical stages and pathological grades shows no significant difference(P>0.05).Conclusions:1.The positive expression of CK13 in normal cervical epithelium, CIN and SCC is gradually decreased, and it has obvious difference, the loss expression of CK13 can be used as a symbol of CINIII and SCC.2.The expression of p63 protein in CIN and SCC is significantly higher than that in normal cervical tissues but it has no significant differences in the expression CIN III of SCC.3.P63 and CK13 were negatively correlated in cervical intraepithelial neoplasia and cervical carcinoma, both of them may be ideal symbol for the development of cervical carcinoma markers.