| Objective: By detecting the mi RNA-107 expression in kidney tissue and normal kidney tissues, To explore the relationship between its expression and kidney cancer cases. Analysis of the mi RNA- 107 mimics influence on 786-0 kidney cancer cell line proliferation, To explore the role of micrornas- 107 in kidney development.Methods: According to the preliminary experiment using the mi RNA expression profile chip detection system screening out the expression differences of kidney cancer and normal renal tissues of mi RNAs,. Validates the differences of four groups of micrornas. Using fluorescence quantitative PCR technique to detect the mi RNA-107 expression in 20 cases of fresh renal clear cell carcinoma tissue and paired normal renal tissues, to explore the relationship between renal clear cell carcinoma and clinical features. In the 786-0 kidney cancer cell line transfect the mi RNA-107 mimics to Simulate the mature cells endogenous mi RNA- 107 high level expressions. By scratch test, MTS colorimetry analysis the mi RNA- 107 mimics influence on cell migration and proliferationResults: The expression of mi RNA- 107 in clear cell carcinoma tissue is far lower than the matched normal renal tissues(P <0.05), the differences between the two groups are significant. The mi RNA-107 expression level in kidney tissues is related to TNM stage and grade of Fuhrman(P<0.05).The difference between mi RNA- 107 and gender, tumor location, size, age had no statistical significance; The mi RNA-107 mimics significantly inhibited 786-0 kidney cancer cell lines growth(P<0.05).Conclusion: Found that the occurrence,development of kidney cancer may be associated with the mi RNA-107, the mi RNA-107 mimics can inhibit renal cancer cell proliferation, migration, suggesting the mi RNA-107 May be as important tumorsuppressor factors involved in inhibition the renal cancer cell proliferation and migration. The mi RNA- 107 could be a kidney cancer diagnosis, treatment targets. |
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