The Relationship Of Vascular Endothelial Growth Factor And Endometrial Receptivity In IVF Patients

Objective : endometrium in the menstrual cycle is only for a period of time to allow the embryo implantation, generally for corpus luteum period, it was a strict time and space limitations, are generally only for 6 to 8 days after ovulation, lasting less than 48 hours, also known as endometrial "planting window" clinically. In vitro fertilization and embryo transplantation(in vitro fertilization and embryo transfer, IVF- ET) technology provides infertility patients with the Gospel, but in clinical practice, even if the application of high technology to screen the relative quality of fertilized egg, embryo transfer success rate is still not high. This topic through the research proposed line of IVF- ET women cultivated window period of vascular endothelial growth factor(vascular endothelial growth factor, VEGF) in endometrial glandular epithelium, cavity epithelial and mesenchymal expression, comparison of VEGF in IVF- ET pregnancy group and IVF- ET before pregnancy way a ovulation cycle secretory endometrium expression level, VEGF and the relationship between the receptivity of endometrium, and VEGF could predict the receptivity of endometrium of indicators to guide clinical.At present there are many methods of evaluation and the receptivity of endometrium, uterine cavity lavage fluid, the determination of the cervical mucus biomarkers, blood express determination of specific hormones and genetic markers at the present stage also have application, but the lack of appropriate accurate and sensitive indicators or expensive, high technical requirements. Endometrial biopsy and the related biological indicators and then organize is clinical recognized evaluation methods, using no pain in patients with endometrial sampler, damage small.Methods : collect the fourth affiliated hospital of reproductive center in hebei medical university to line 7 days after ovulation IVF- ET women plantwindow phase of endometrium? Example, using immunohistochemical SP method detection: the endometrium after 4% formaldehyde fixed paraffin embedding, preparation4 microns thick continuous biopsy.Immunohistochemical staining. Dewaxing, high-pressure antigen repair,closed, add a resistance(VEGF < R159 > polyclonal antibody dilution concentration of 1:100), two resistance, DAB color, wood grain redyeing,dehydrated through neutral gum after sealing piece. Use PBS instead of a negative resistance to do comparison, microscope and photographed. Positive judgment standard: each dyeing piece randomly selected five horizons, with clear background, cytoplasmic staining in tan for positive, divided into four levels: no positive staining cells were for "-". 25 the following cells positive staining for weakly positive(+); 25% ~ 50% cells positive staining for moderate positive(+ +); More than 50% of the cells positive staining strong positive(+ + +). Each application rt-pcr detection technology, the research group and control group in endometrial approximately 50 mg Rezol 1 ml homogenate in fully homogenate, extracting total RNA, remove the 0.5 mu l,reverse transcription c DNA, which in turn to PCR amplification, at the same time with amplified fragment length is 605 bp GAPDH as reference. VEGF PCR circulation condition is: 95 10 min ℃, 1 cycle; 95 45 ℃ s, 60 s 45 ℃,72 ℃, 50 s 35 cycles; 10 min, 72 ℃, 1 cycle. Ang2 PCR circulation condition is: 95 10 min ℃, 1 cycle; 95 45 ℃ s, 60 s 45 ℃, 72 ℃, 50 s 35 cycles; 10 min,72 ℃, 1 cycle. Product after 1.5% agarose gel electrophoresis, with VDS photographic image analysis system and image analysis. IOD values within the reference correction, distribution in statistical analysis. According to whether pregnancies after IVF- ET will? Patients with infertility were divided into two groups: pregnancy(cases) and not the pregnancy group(cases). Use support SPSS11.5 software package for processing data, measurement data with mean + /- standard deviation( x + s) said, mean comparison between the two groups using independent sample t test and inspection level at P < 0.05 for the difference is statistically significant.Results :1 the average age of both groups respectively?27.36±3.18 And age28.41±4.15 Fixed number of year of the age, the age difference between the two groups, infertility, the number of eggs and embryos that designed.the Gn days, count, there were no statistical significance(p < 0.05).2 immune detection found that planting window period of VEGF in positive expression in endometrial cavity epithelium and gland, located in cytoplasm, and apocrine shaped, also visible within the glandular lumens are known as tan material formation, glands expressed a strong cavity epithelium.3 IVF- ET in women without pregnancy group the endometrial VEGF expression was lower than those of pregnancy group.Conclusion :1 endometrial cavity epithelium and glands VEGF window is grown in the embryonic period of strong expression suggests VEGF plays an important role in embryo implantation.2 IVF- ET in women without pregnancy group the endometrial VEGF expression was lower than those of pregnancy group, the prompt endometrial VEGF may be affect the receptivity of endometrium, and one of the reasons why the implantation rate.3 the determination of VEGF expression in the lining of the uterus can be used as predict IVF- ET one of the reference index of pregnancy success.