On The Correlation Of The Successes Of Embryo Implantation With The Expression Of LIF And Integrin A_vβ₃ In Human Endometrium Within The Implantation Window

Objective:To determine the expressions of the endometrial leukemia inhibitory factor(LIF)and Integrinανβзin the endometrium and their correlation with the embryo implantation.Methods:The endometrium samples were obtained during the implantation window from 42 patients undergoing IVF-ET.The endometrial LIF and Integrinανβзwere detected by immunohistochemistry using polyclonal antibody and semi-quantified by H-score.The 42 patients were divided into two groups according to the clinical outcomes:the pregnancy group(14 patients)and non-pregnancy group(28 patients). The expression of endometrial leukemia inhibitory factor(LIF)and Integrinανβзin the endometrium was studied and their relationship with Embryo Implantation outcome was investigated.Results:1.The LIF expresses in the cytoplasm of the luminal epithelial and glandular cells during the mid-stage of secretion.The H-scores from LIF immunostaining of luminal epithelial and glandular were significantly higher in the pregnant group than those in the non-pregnant group with statistical significance(P＜0.05).2.Integrinανβзexpresses in the the luminal epithelial and glandular cells during the mid-stage of secretion.The H-scores from Integrinανβзimmunostaining of luminal epithelial and glandular were significantly higher in the pregnant group than those in non-pregnant group with statistical significance(P＜0.01).3.Statistical difference(P＜0.05)was observed between the pregnant and the nonpregnant groups in the positive rates of the expression of LIF and Integrinανβзin the endometrium during the mid-stage of the secretion.Conclusions:1.LIF and Integrinανβзwas expressed in a specific stage of endometrial development,that is,the seventh day after ovulation,which is the embryo implantation window.2.The H-scores from LIF and Integrinανβзimmunostaining of endometrium epithelial were significantly higher in the pregnancy group than those in the nonpregnancy group,indicating that LIF and Integrinανβзare important for the formation of the implantation window of the endometrium.3.The enhanced expression of LIF and Integrinανβзin the endometrium during the mid-stage of the secretion can be employed as one of the criteria to predict the receptivity of the endometrium.